Apical Secretion of FSTL1 in the Respiratory Epithelium for Normal Lung Development.
Apical Secretion of FSTL1 in the Respiratory Epithelium for Normal Lung Development.
复制标题
呼吸道上皮中 FSTL1 的顶端分泌对正常肺发育的影响
DOI：
10.1371/journal.pone.0158385
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Ning W
中科院分区：
文献类型：
--
作者：
Li X;Fang Y;Li X;Liang J;Jiang D;Geng Y;Ning W
卵泡抑素样蛋白1（FSTL 1）是一种分泌型骨形态发生蛋白（BMP）拮抗剂，在肺的正常发育中起着至关重要的作用。Fstl 1的缺失导致小鼠出生后因呼吸衰竭而死亡。为了进一步探索FSTL 1在小鼠肺发育中的作用，我们创建了转基因SFTPC-Fstl 1等位基因小鼠，其在肺发育的所有阶段均显示Fstl 1的显著上皮过表达。然而，上皮过表达Fstl 1并不改变肺形态发生，上皮分化和肺功能。此外，我们发现FSTL 1功能被上皮极化所阻断，这反映在FSTL 1的显著顶端分泌和基底外侧BMP信号传导。总之，这项研究表明，FSTL 1和BMP信号的紧密空间相互作用在肺发育中起着至关重要的作用。
Follistatin-like 1 (FSTL1) is a secreted bone morphogenetic protein (BMP) antagonist, and it plays a crucial role in normal lung development. Deletion of Fstl1 leads to postnatal death in mice due to respiratory failure. To further explore the role of FSTL1 in mouse lung development, we created a transgene SFTPC-Fstl1 allele mouse displaying significant epithelial overexpression of Fstl1 in all stages of lung development. However, epithelial overexpression of Fstl1 did not alter lung morphogenesis, epithelial differentiation and lung function. Moreover, we found that FSTL1 function was blocked by the epithelial polarization, which was reflected by the remarkable apical secretion of FSTL1 and the basolateral BMP signaling. Taken together, this study demonstrates that tightly spatial interaction of FSTL1 and BMP signaling plays an essential role in lung development.