Expression of P-glycoprotein in hepatocellular carcinoma: A potential marker of prognosis
Expression of P-glycoprotein in hepatocellular carcinoma: A potential marker of prognosis
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DOI：
10.1136/jcp.49.6.470
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发表时间：
1996-06-01
影响因子：
3.4
通讯作者：
Paakko, P
中科院分区：
文献类型：
--
作者：
Soini, Y;Virkajarvi, N;Paakko, P
目的(1)研究多药耐药基因（MDR1）产物p -糖蛋白在31例肝细胞癌（hcc）组织标本中的免疫组织化学表达；(2)将结果与细胞增殖、p53表达、无病期和患者累积生存期联系起来。方法采用亲和素-生物素过氧化物酶法，分别用单克隆抗体- c219、兔多克隆抗人抗体CM-1和小鼠单克隆抗人抗体PC10检测p -糖蛋白、p53和增殖细胞核抗原（PCNA）的表达。结果：31例hcc中p -糖蛋白膜结合阳性20例（65%）。细胞质球状阳性也见于部分病例。p -糖蛋白的表达与细胞增殖（由PCNA免疫表达和有丝分裂计数确定）或p53表达之间无显著关联。p -糖蛋白阳性肿瘤患者的无病间期较p -糖蛋白阴性肿瘤患者短，生存时间也较短。p -糖蛋白阳性患者接受或未接受化疗的生存率没有差异，这表明化疗（主要是丝裂霉素- c）不会影响这些患者的生存。结论：肝细胞癌中p -糖蛋白的表达与较短的无病期和较短的生存期有关。由于p -糖蛋白的表达与细胞增殖或p53的表达无关，其对疾病进展和生存的影响似乎与这些因素无关。
Aims-(1) To investigate the immunohistochemical expression of the multidrug resistance gene (MDR1) product P-glycoprotein in histological samples from 31 hepatocellular carcinomas (HCCs); and (2) to correlate the results with cell proliferation, p53 expression, the disease-free interval, and cumulative patient survival.Methods-C219 (a monoclonal antibody), CM-1 (a polyclonal rabbit anti-human antibody) and PC10 (a monoclonal mouse anti-human antibody) were used to detect expression of P-glycoprotein, p53 and proliferating cell nuclear antigen (PCNA), respectively, by means of the avidin-biotin peroxidase method.Results-Membrane bound positivity for P-glycoprotein was observed in 20 (65%) of the 31 HCCs. Cytoplasmic globular positivity was also seen in some cases. There were no significant associations between expression of P-glycoprotein and cell proliferation (determined by PCNA immunoexpression and the mitotic count), or p53 expression. Patients with P-glycoprotein positive tumours had a shorter disease-free interval than those with P-glycoprotein negative tumours, and also had a shorter survival time. There was no difference in survival between P-glycoprotein positive patients who had or had not received chemotherapy suggesting that chemotherapy (mainly mitomycin-C) did not affect survival in these patients.Conclusions-Expression of P-glycoprotein in HCCs is associated with a shorter disease-free interval and shorter survival time. As expression of P-glycoprotein was not associated with cell proliferation or expression of p53, its effect on disease progression and survival seems to be independent of these factors.