GFAP MESSENGER-RNA INCREASES WITH AGE IN RAT AND HUMAN BRAIN
GFAP MESSENGER-RNA INCREASES WITH AGE IN RAT AND HUMAN BRAIN
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DOI：
10.1016/0197-4580(93)90100-p
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发表时间：
1993-09-01
影响因子：
4.2
通讯作者：
FINCH, CE
中科院分区：
文献类型：
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作者：
NICHOLS, NR;DAY, JR;FINCH, CE
用RNA印迹杂交法检测了两个队列的三个年龄组的雄性F-344大鼠和47例人死后脑组织中胶质细胞酸性蛋白（GFAP）mRNA的表达。GFAP mRNA在24只大鼠海马和纹状体中的表达高于6- 7月龄大鼠。另一个星形胶质细胞的分子标记，谷氨酰胺合成酶mRNA，并没有随着年龄的变化，在大鼠脑。大鼠GFAP mRNA表达率与血清睾酮呈负相关，但与血清皮质酮无相关性。在人类海马、额叶和颞叶皮层中，GFAP mRNA在老年人（60-79岁）中也比中年人（25-59岁）增加。与此相反，线粒体细胞色素氧化酶亚基1 mRNA在任何地区的年龄组之间没有变化。通过结合这三个区域进行进一步分析，GFAP mRNA随着年龄的增长而增加，无论性别，中年组的酗酒，或者大脑是否被归类为正常或神经病理（不包括阿尔茨海默病病理）。这些数据表明，增加GFAP蛋白或GFAP-免疫反应性星形胶质细胞在大鼠和人类可能会导致转录或转录后调节，并扩展到三个物种（包括小鼠）显示随着年龄的增长GFAP mRNA的增加。已知调节年轻大脑中GFAP mRNA表达的因素被认为是年龄相关性增加的可能原因。
Glial fibrillary acidic protein (GFAP) mRNA was examined by RNA blot hybridization in three age groups of two cohorts of male F-344 rats and in 47 human postmortem brain samples. GFAP mRNA increased in the hippocampus and striatum of 24 versus 6- to 7-month-old rats. Another astrocytic molecular marker, glutamine synthetase mRNA, did not change with age in rat brain. Rat GFAP mRNA prevalence was inversely correlated with serum testosterone but not correlated with serum corticosterone. In human hippocampus, frontal and temporal cortex, GFAP mRNA also increased in older (60-79 years) compared with middle-aged (25-59 years) individuals. In contrast, mitochondrial cytochrome oxidase subunit 1 mRNA did not change between age groups in any region. By combining the three regions for further analysis, GFAP mRNA increased with age irregardless of gender, alcoholism in the middle-aged group, or whether brains were classified as normal or neuropathologic (excluding Alzheimer's disease pathology). These data indicate that increased GFAP protein or GFAP-immunoreactive astrocytes in rats and humans may result from transcriptional or post-transcriptional regulation and extend the number to three species (including mouse) showing an increase in GFAP mRNA with age. Factors that are known to regulate GFAP mRNA expression in young brains are considered as possible causes of age-related increases.