Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia
Aedes albopictus (Skuse, 1894) infected with the American-Asian genotype of dengue type 2 virus in Medellín suggests its possible role as vector of dengue fever in Colombia
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DOI：
10.7705/biomedica.v34i2.3474
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发表时间：
2017-09-01
期刊：
影响因子：
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通讯作者：
Uribe, Sandra
中科院分区：
文献类型：
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作者：
Gómez-Palacio, Andrés;Suaza-Vasco, Juan;Uribe, Sandra
简介：埃及伊蚊和伊蚊。白纹伊蚊是世界上一些国家公认的登革热、黄热病、基孔肯雅病和寨卡虫媒病毒的媒介。在哥伦比亚，Ae。白纹伊蚊的地理分布已扩大到包括卡利和麦德林等人口稠密的城市。虽然在该国的城市和半城市地区经常发现该物种，但对其作为登革热媒介的作用知之甚少。目的：鉴别中药伊蚊的存在。在麦德林收集的自然感染登革热病毒的白纹伊蚊标本。材料与方法：昆虫采集于哥伦比亚国立大学麦德林校区。个体分类为伊蚊；白纹伊蚊DNA条形码区分析证实。对蚊虫进行登革病毒鉴定，对NS3基因片段进行测序，并与文献报道的DENV-2基因型进行比较。结果：COI序列分析显示Ae；白纹伊蚊个体与最近在哥伦比亚报告的白纹伊蚊个体相似，在遗传上与世界其他地区的白纹伊蚊个体接近。在测试的池中，有一个对DENV-2呈阳性，NS3分析表明它属于亚裔美国人的分支。结论：我们报道了Ae。哥伦比亚的白纹伊蚊自然感染了DENV-2的亚裔美国基因型。Ae的存在。在麦德林等人口稠密的城市携带最常见基因型感染人类的白纹伊蚊标本表明，它在哥伦比亚可能发挥登革热媒介的作用，并突出了将其纳入当前媒介监测战略的相关性。
INTRODUCTION: Aedes aegypti and Ae. albopictus are recognized vectors of dengue, yellow fever, chikungunya and Zika arboviruses in several countries worldwide. In Colombia, Ae. albopictus geographical distribution has increased to include highly populated cities such as Cali and Medellin. Although this species has been frequently found in urban and semi-urban zones in the country, its role as vector of the dengue fever is poorly known.OBJECTIVE: To identify the presence of Ae. albopictus specimens naturally infected with dengue virus collected in Medellin.MATERIALS AND METHODS: Insects were collected in the Universidad Nacional de Colombia campus in Medellin. Individuals were classified as Ae. albopictus and confirmed by DNA barcode region analysis. Mosquitoes were processed for dengue virus identification, and a fragment of the NS3 gen was sequenced and compared with DENV-2 genotypes reported in the literature.RESULTS: Sequence analysis of COI indicated Ae. albopictus individuals were similar to those recently reported in Colombia, and genetically close to those from other regions worldwide. Among the pools tested one was positive for DENV-2, and the NS3 analysis indicated it belonged to the Asian-American clade.CONCLUSION: We report the presence Ae. albopictus naturally infected with the Asian-American genotype of DENV-2 in Colombia. The presence of Ae. albopictus specimens carrying the most common genotype infecting humans in a highly populated city such as Medellin indicates its potential role as dengue vector in Colombia and highlights the relevance of including it in current vector surveillance strategies.