Sensitive detection of Bacillus anthracis using a binding protein originating from γ-phage
Sensitive detection of Bacillus anthracis using a binding protein originating from γ-phage
复制标题
DOI：
10.1111/j.1348-0421.2007.tb03894.x
复制
发表时间：
2007-01-01
影响因子：
2.6
通讯作者：
Yasuda, Jiro
中科院分区：
文献类型：
--
作者：
Fujinami, Yoshihito;Hirai, Yoshikazu;Yasuda, Jiro
生物武器的探测是部队保护、条约核查和保护平民免受国内恐怖主义侵害的首要问题。最令人担忧的是炭疽杆菌的检测，炭疽杆菌是炭疽的病原体。因此，迫切需要开发快速、简便、精确检测炭疽芽胞杆菌的新方法。在这里，我们报道了γ -噬菌体裂解蛋白（PlyG）的c端区域特异性结合到炭疽杆菌的细胞壁上，并且该区域对应的重组蛋白（位置，156-233）PlyGB可作为检测炭疽杆菌的生物探针。我们的检测方法基于重组PlyGB的膜直接印迹法，比噬菌体检测更快、更敏感，比PCR等遗传方法或使用特异性抗体的免疫学方法更简单、更便宜。此外，其特异性与γ -噬菌体试验相当。PlyGB可用于常规方法而不是抗体，可能成为检测炭疽杆菌的有效工具。
Detection of biological weapons is a primary concern in force protection, treaty verification, and safeguarding civilian populations against domestic terrorism. One great concern is the detection of Bacillus anthracis, the causative agent of anthrax. Therefore, there is a pressing need to develop novel methods for rapid, simple, and precise detection of B. anthracis. Here, we report that the C-terminal region of gamma-phage lysin protein (PlyG) binds specifically to the cell wall of B. anthracis and the recombinant protein corresponding to this region (positions, 156-233), PlyGB, is available as a bioprobe for detection of B. anthracis. Our detection method, based on a membrane direct blot assay using recombinant PlyGB, was more rapid and sensitive than they-phage test and was simpler and more inexpensive than genetic methods such as PCR, or immunological methods using specific antibodies. Furthermore, its specificity was comparable to the gamma-phage test. PlyGB is applicable in conventional methods instead of antibodies and could be a potent tool for detection of B. anthracis.