Structural and functional evidence for multiple channel complexes in the outer membrane of Neurospora crassa mitochondria.
Structural and functional evidence for multiple channel complexes in the outer membrane of Neurospora crassa mitochondria.
复制标题
粗糙脉孢菌线粒体外膜中多通道复合物的结构和功能证据。
DOI：
10.1073/pnas.80.8.2243
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
Frank,J
中科院分区：
文献类型：
--
作者：
Mannella,CA;Colombini,M;Frank,J
The outer membrane of mitochondria contains proteins that form channels called VDAC (voltage-dependent anion-selective channels). Two independent lines of evidence suggest that these channels occur in specific complexes in the outer membrane of Neurospora mitochondria. Electron microscopic images of these outer membranes reveal polymorphic crystalline arrays of putative pores. These arrays can be shown to be interrelated by movement in the membrane plane of a particular rigid channel triplet or of regular aggregates of this triplet. Detergent extracts of the same outer membranes induce single- and multiple-step conductances in planar phospholipid membranes, with a marked preference for the insertion of triplets and multiples of triplets. The tendency of the mitochondrial channels to occur as extended arrays, apparently built up from triplets, may have important functional and evolutionary implications.
