Molecular evidence for hybrid origin of Melastoma intermedium
Molecular evidence for hybrid origin of Melastoma intermedium
复制标题
中间野牡丹杂交起源的分子证据
DOI：
10.1016/j.bse.2011.12.010
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发表时间：
2012-04-01
影响因子：
1.6
通讯作者：
Zhou, Renchao
中科院分区：
文献类型：
--
作者：
Dai, Seping;Wu, Wei;Zhou, Renchao
在本研究中，我们对中国特有的灌木中间野牡丹及其可能的亲本野牡丹和十二枝野牡丹的两个核基因和一个叶绿体间隔区进行了测序，以验证杂交起源假说。我们的结果表明，在一个核基因中，白念珠菌和十二枝链霉间存在5个固定的核苷酸替换，而在另一个核基因中，有6个核苷酸替换。除1个个体外，所有中间分枝杆菌在至少一个基因的这些位点上都表现出相加性。在这两个核基因上，念珠菌和十二枝菌的单倍型分离得很好，中间偃麦草的大多数单倍型与念珠菌和十二枝菌的单倍型是相同的。白念珠菌和十二枝莲在叶绿体间隔区有三个核苷酸替换，而中间锦鸡儿的个体与白念珠菌或十二枝锦鸡儿的序列相同。分子数据清楚地表明中间分枝杆菌是杂交起源的。(C)2011爱思唯尔有限公司。保留所有权利。
In this study, we sequenced two nuclear genes and one chloroplast spacer in Melastoma intermedium, a shrub species endemic to China, and its putative parental species, Melastoma candidum and Melastoma dodecandrum, to test the hybrid-origin hypothesis. Our results revealed that in one nuclear gene there were five fixed nucleotide substitutions between M. candidum and M. dodecandrum, and in the other nuclear gene, there were six. All but one individual of M. intermedium showed additivity in chromatograms at these sites of at least one gene. Haplotypes of M. candidum and M. dodecandrum at the two nuclear genes were well separated, and most haplotypes of M. intermedium were shared with those of M. candidum and M. dodecandrum. M. candidum and M. dodecandrum differed by three nucleotide substitutions in the chloroplast spacer, whereas individuals of M. intermedium had identical sequences to either M. candidum or M. dodecandrum. The molecular data clearly demonstrate that M. intermedium is of hybrid origin. (C) 2011 Elsevier Ltd. All rights reserved.