Role of Annexin A2 isoform 2 on the aggregative growth of dermal papillae cells
Role of Annexin A2 isoform 2 on the aggregative growth of dermal papillae cells
复制标题
膜联蛋白 A2 异构体 2 对真皮乳头细胞聚集生长的作用
DOI：
10.1042/bsr20180971
复制
发表时间：
2018-12-21
影响因子：
4
通讯作者：
Xia, Rushan
中科院分区：
文献类型：
--
作者：
Gu, Jing;Ma, Yinni;Xia, Rushan
毛乳头是头发的主要成分，它向毛囊上皮细胞发出信号，以延长头发的生长过程。通过双向凝胶电泳法(2-DE)、MALDI-TOF-MS和数据库检索对人Annexin A2进行了初步鉴定。本研究旨在探讨Annexin A2在毛乳头细胞(DPC)聚集生长中的作用。逆转录-聚合酶链式反应(RT-PCR)和Western印迹法检测Annexin A2的表达。用siRNA技术抑制Annexin A2的表达。构建过表达载体上调Annexin A2的表达。用细胞计数试剂盒8(CCK-8)和增殖细胞核抗原(PC NA)检测DPC的增殖情况。RT-PCR结果显示，与第10代相比，第3代DPC的Annexin A2基因表达上调。Western印迹分析表明，与第10代相比，第3代DPC中Annexin A2亚型2的表达显著上调，与基因水平上的结果一致。合成Annexin A2异构体2 siRNA，并将其导入第3代DPC。RT-PCR结果显示，在第3代DPC中Annexin A2亚型2的mRNA表达受到抑制。Western印迹结果显示，在第3代DPC中Annexin A2亚型2和增殖细胞核抗原的表达水平受到抑制。CCK-8结果显示，第3代DPC的增殖受到抑制(P
The dermal papilla is a major component of hair, which signals the follicular epithelial cells to prolong the hair growth process. Human Annexin A2 was preliminarily identified by two-dimensional gel electrophoresis (2-DE), MALDI-TOF-MS and database searching. The aim of the present study was to explore the role of Annexin A2 in the aggregative growth of dermal papillae cells (DPC). Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were adopted to detect the expression of Annexin A2. And siRNA technique was used to suppress the expression of Annexin A2. Construction of over-expression vector was used to up-regulate the expression of Annexin A2. Cell Counting Kit 8 (CCK-8) and proliferating cell nuclear antigen (PCNA) were taken to detect the proliferation of DPC. The expression of Annexin A2 mRNA was up-regulated in passage 3 DPC compared with passage 10 DPC by RT-PCR. In line with the results at the mRNA level, Western blot analysis revealed that Annexin A2 isoform 2 was up-regulated significantly in passage 3 DPC compared with passage 10 DPC. The Annexin A2 isoform 2 siRNA was synthesized and transfected into passage 3 DPC. RT-PCR data showed the mRNA expression of Annexin A2 isoform 2 was suppressed in passage 3 DPC. Western blot results showed the expression level of Annexin A2 isoform 2 and PCNA were suppressed in passage 3 DPC. CCK-8 results showed that the proliferation of passage 3 DPC was suppressed (P