CO2‐sensitive medullary neurons: activation by intracellular acidification
CO2‐sensitive medullary neurons: activation by intracellular acidification
复制标题
CO2 敏感的髓质神经元：通过细胞内酸化激活
作者：
M. Wiemann;R. E. Baker;U. Bonnet;D. Bingmann
在BCECF-AM加载培养物中测量的细胞内pH（pHi）的变化期间，检测新生大鼠奥贝水平器官型培养物中CO2敏感的腹外侧髓神经元（VLNCS）的生物电活性。高碳酸血症（pCO 2 80-100 mmHg）使pHi降低0.15 ± 0.06单位，并刺激神经元放电。用HEPES（26 mM，pH 7.4）置换浴中的CO2/HCO 3 − 10 min，酸化pHi（0.07 ± 0.03单位），并激发VLNCS。氯化铵（10 mM，1 min）首先碱化（0.1 ± 0.04），然后酸化pHi（0.06 ± 0.03），而细胞外pH首先酸化，然后碱化。这导致神经元放电首先被抑制，然后被加速。研究结果强烈表明，细胞内而不是细胞外酸化激活CO2敏感神经元。
BIOELECTRIC activity of CO2-sensitive, ventrolateral medullary neurons (VLNCS) in organotypic cultures from the obex level of newborn rats was tested during changes of the intracellular pH (pHi) measured in BCECF-AM loaded cultures. Hypercapnia (pCO2 80–100 mmHg) reduced pHi by 0.15 ± 0.06 units and stimulated neuronal discharges. Replacement of CO2/HCO3− in the bath by HEPES (26 mM, pH 7.4) for 10 min acidified pHi (0.07 ± 0.03 units) and also excited VLNCS. Ammonium chloride (10 mM, 1 min) initially alkalized (0.1 ± 0.04) and thereafter acidified pHi (0.06 ± 0.03), while the extracellular pH was first acidified and then alkalized. This resulted in neuronal discharge which were first suppressed and then accelerated. The findings strongly suggest that intracellular rather than extra-cellular acidification activates CO2-sensitive neurons.
脑池Na转运抑制和对CO2的通气反应。
DOI：
10.1152/jappl.1994.77.6.2572
发表时间：
1994
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Sullivan,MP;Adams,JM
通讯作者：
Adams,JM
大鼠海马切片中活动依赖性 pH 值变化的时间分辨率。
影响因子：
2.5
作者：
Gottfried,JA;Chesler,M
通讯作者：
Chesler,M