A recessive high-density pod mutant resource of Brassica napus
A recessive high-density pod mutant resource of Brassica napus
复制标题
甘蓝型油菜隐性高密度荚果突变体资源
DOI：
10.1016/j.plantsci.2020.110411
复制
发表时间：
2020-04-01
期刊：
影响因子：
5.2
通讯作者：
Xiang, Yang
中科院分区：
文献类型：
--
作者：
Tang, Minqiang;Tong, Chaobo;Xiang, Yang
在甘蓝型油菜中，荚果数量和荚果密度是决定种子产量的关键因素。尽管荚果密度是一个重要的产量性状，但人们对油料作物产量形成的调控以及遗传和分子机制知之甚少。在这项研究中，我们通过复合杂交鉴定了油菜高密度荚果突变体（dpt247）。为了阐明这种突变的本质，我们从形态学、解剖学、生理学、遗传学和转录组学上对其进行了研究。突变体植株与对照植株相比表现出明显的表型差异，包括株高和初生分枝长度降低，初生分枝数量减少，主花序上的荚果数量显着增加，荚果分布更加紧凑。此外，突变体的茎尖分生组织（SAM）中吲哚-3-乙酸（IAA）和玉米素核苷（ZR）的含量较高。密集的荚果性状由 GRE501 和 dpt247 分离遗传群体中鉴定的两个主要隐性基因控制。 RNA测序表明dpt247中参与生长素、细胞分裂素和WUS/CLV信号通路的基因在突变体中更加活跃。这些结果为了解油菜产量形成调控和高产育种提供了重要信息。
In Brassica napus, pod number and pod density are critical factors to determine seed yield. Although the pod density is an essential yield trait, the regulation of yield formation in oil crops, as well as the genetic and molecular mechanisms, are poorly understood. In this study, we characterized a rapeseed high-density pod mutant (dpt247) from composite hybridization. To shed some light on the nature of this mutation, it was investigated morphologically, anatomically, physiologically, genetically and transcriptomically. The mutant plant showed noticeable phenotypic differences in comparison with the control plant, including reduced plant height and primary branch length, decreased number of primary branches, significantly increased number of pod on the main inflorescence, and more compact pod distribution. Besides, the mutant had higher levels of indole-3-acetic acid (IAA) and zeatin riboside (ZR) in the shoot apical meristem (SAM). The dense pod trait was controlled by two major recessive genes identified in the segregating genetic populations of GRE501 and dpt247. RNA sequencing indicated genes participated in auxin, cytokinin and WUS/CLV signalling pathway in dpt247 were more active in the mutant. These results provide important information for understanding the regulation of yield formation and high yield breeding in rapeseed.