Survival trade-offs in plant roots during colonization by closely related beneficial and pathogenic fungi.
Survival trade-offs in plant roots during colonization by closely related beneficial and pathogenic fungi.
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DOI：
10.1038/ncomms11362
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发表时间：
2016-05-06
影响因子：
16.6
通讯作者：
O'Connell RJ
中科院分区：
文献类型：
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作者：
Hacquard S;Kracher B;Hiruma K;Münch PC;Garrido-Oter R;Thon MR;Weimann A;Damm U;Dallery JF;Hainaut M;Henrissat B;Lespinet O;Sacristán S;Ver Loren van Themaat E;Kemen E;McHardy AC;Schulze-Lefert P;O'Connell RJ
植物的秘密性质迫使它们发展机制以优先考虑其对同时应激的反应，包括微生物或营养饥饿。在这里，我们比较了有益的根部内生菌，colletotrichum tofieldiae及其致病性相对梭菌的基因组，并检查真菌及其植物的转录组在磷酸盐饥饿期间的转录组。尽管这两个物种仅在880万年前差异，并且具有相似的基因武库，但我们确定了基因称特征，表明了从致病性到有益生活方式的进化过渡，包括狭窄的分泌效应蛋白的曲目，扩展的甲壳质结合和二级代谢 - 代谢 - 代谢性的家族 - 相关蛋白质，植物学中与致病性相关基因的激活有限。我们表明，在缺乏磷酸盐的条件下，在tofieldiae-cole殖民化的根中优先考虑有益反应，而在磷酸盐充足的条件下，防御反应被激活。这些免疫反应保留在通过致病性梭菌殖民的磷酸盐饥饿的根中，这说明了植物对响应冲突的应激的最大生存的能力。
colletotrichum tofieldiae是一种有益的根部内生菌，而密切相关的关联是致病性的。在这里，作者比较了宿主植物相互作用期间的基因组和转录组，并证明宿主植物根据磷酸盐状态对有益的内生菌反应有所不同。
The sessile nature of plants forced them to evolve mechanisms to prioritize their responses to simultaneous stresses, including colonization by microbes or nutrient starvation. Here, we compare the genomes of a beneficial root endophyte, Colletotrichum tofieldiae and its pathogenic relative C. incanum, and examine the transcriptomes of both fungi and their plant host Arabidopsis during phosphate starvation. Although the two species diverged only 8.8 million years ago and have similar gene arsenals, we identify genomic signatures indicative of an evolutionary transition from pathogenic to beneficial lifestyles, including a narrowed repertoire of secreted effector proteins, expanded families of chitin-binding and secondary metabolism-related proteins, and limited activation of pathogenicity-related genes in planta. We show that beneficial responses are prioritized in C. tofieldiae-colonized roots under phosphate-deficient conditions, whereas defense responses are activated under phosphate-sufficient conditions. These immune responses are retained in phosphate-starved roots colonized by pathogenic C. incanum, illustrating the ability of plants to maximize survival in response to conflicting stresses. Colletotrichum tofieldiae is a beneficial root endophyte, whereas the closely related C. incanum is pathogenic. Here the authors compare the genomes and transcriptomes during host plant interaction and demonstrate that the host plant can respond differently to the beneficial endophyte according to phosphate status.