The UBX-regulated network in the haltere imaginal disc of D-melanogaster
The UBX-regulated network in the haltere imaginal disc of D-melanogaster
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DOI：
10.1016/j.ydbio.2006.11.011
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发表时间：
2007-02-15
影响因子：
2.7
通讯作者：
Carroll, Sean B.
中科院分区：
文献类型：
--
作者：
Hersh, Bradley M.;Nelson, Craig E.;Carroll, Sean B.
HOX蛋白被认为在调控体系中的多个水平上起作用，并直接控制过多的靶基因的表达。然而，对于任何特定的HOX蛋白或组织，很少有直接在体内调节的靶基因被识别出来。在这里，我们已经确定了Hex蛋白Ultrabithorax(UBx)的目标基因，它修改了翅膀的遗传调控网络，以产生Hatere，一种改进的后翼。我们使用全基因组微阵列和定制阵列，包括所有预测的黑腹果蝇基因组中的转录因子和信号分子，以确定翅膀和龟头成像盘中差异表达的基因。为了更详细地阐明所选基因的调控，我们分离了在翼盘或缰盘中特异表达的基因的顺式调节元件(CRE)。我们证明了UBX直接与一个元素中的位点结合，这些位点对于卤化物盘中的激活至关重要。这些结果表明，缰节和后胸节的形态不是仅仅通过关闭翅膀和间胸发育程序来实现的，而是必须激活特定的基因来形成这些结构。Hatere形态的进化涉及整个翅膀遗传调控网络中Ubx调控的靶基因的变化，包括正向和负向基因。(C)2006 Elsevier Inc.保留所有权利。
Hox proteins have been proposed to act at multiple levels within regulatory hierarchies and to directly control the expression of a plethora of target genes. However, for any specific Hox protein or tissue, very few direct in vivo-regulated target genes have been identified. Here, we have identified target genes of the Hex protein Ultrabithorax (UBX), which modifies the genetic regulatory network of the wing to generate the haltere, a modified hindwing. We used whole-genome microarrays and custom arrays including all predicted transcription factors and signaling molecules in the Drosophila melanogaster genome to identify differentially expressed genes in wing and haltere imaginal discs. To elucidate the regulation of selected genes in more detail, we isolated cis-regulatory elements (CREs) for genes that were specifically expressed in either the wing disc or haltere disc. We demonstrate that UBX binds directly to sites in one element, and these sites are critical for activation in the haltere disc. These results indicate that haltere and metathoracic segment morphology is not achieved merely by turning off the wing and mesothoracic development programs, but rather specific genes must also be activated to form these structures. The evolution of haltere morphology involved changes in UBX-regulated target genes, both positive and negative, throughout the wing genetic regulatory network. (c) 2006 Elsevier Inc. All rights reserved.