Electron microprobe analysis of human labial gland secretory granules in cystic fibrosis.
Electron microprobe analysis of human labial gland secretory granules in cystic fibrosis.
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囊性纤维化中人唇腺分泌颗粒的电子探针分析。
DOI：
10.1172/jci111911
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Young,M
中科院分区：
文献类型：
--
作者：
Izutsu,K;Johnson,D;Schubert,M;Wang,E;Ramsey,B;Tamarin,A;Truelove,E;Ensign,W;Young,M
冻干阴唇腺冷冻切片x线显微分析显示，囊性纤维化（CF）患者的阴唇腺分泌颗粒中Na浓度较正常人增加一倍，Ca/S浓度比降低。其他结果表明，Ca/S浓度比的降低是由于S浓度的增加。这些结果表明，唇口唾液黏液颗粒中Na和S含量的增加与cf相关，这种变化可能会影响胞吐后黏液的流变学。与先前对人腮腺的研究相反，没有发现与cf相关的细胞质或细胞核Na、K和Ca浓度变化的证据。分泌颗粒与对照细胞的细胞核和细胞质之间存在显著的元素差异。图片
X-ray microanalysis of freeze-dried labial gland cryosections revealed that Na concentration was doubled and the Ca/S concentration ratio was decreased in secretory granules of labial glands from patients with cystic fibrosis (CF) when compared with glands from normal subjects. Other results suggested that the decrease in the Ca/S concentration ratio resulted from an increase in S concentration. These findings imply that mucous granules in labial saliva showed a CF-related increase in Na and S content, and such changes would be expected to affect the rheology of the mucus after exocytosis. In contrast with a previous study in human parotid glands, no evidence was found for CF-related changes in cytoplasmic or nuclear Na, K, and Ca concentrations. Significant elemental differences were found between secretory granules and nuclei and cytoplasm of control cells.Images