Characterization of chromatin accessibility with a transposome hypersensitive sites sequencing (THS-seq) assay.
Characterization of chromatin accessibility with a transposome hypersensitive sites sequencing (THS-seq) assay.
复制标题
通过转座体超敏位点测序（THS-SEQ）测定的染色质可及性的表征。
DOI：
10.1186/s13059-016-0882-7
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发表时间：
2016-02-04
期刊：
影响因子：
12.3
通讯作者：
Zhang K
中科院分区：
文献类型：
--
作者：
Sos BC;Fung HL;Gao DR;Osothprarop TF;Kia A;He MM;Zhang K
Chromatin accessibility captures in vivo protein-chromosome binding status, and is considered an informative proxy for protein-DNA interactions. DNase I and Tn5 transposase assays require thousands to millions of fresh cells for comprehensive chromatin mapping. Applying Tn5 tagmentation to hundreds of cells results in sparse chromatin maps. We present a transposome hypersensitive sites sequencing assay for highly sensitive characterization of chromatin accessibility. Linear amplification of accessible DNA ends with in vitro transcription, coupled with an engineered Tn5 super-mutant, demonstrates improved sensitivity on limited input materials, and accessibility of small regions near distal enhancers, compared with ATAC-seq. The online version of this article (doi:10.1186/s13059-016-0882-7) contains supplementary material, which is available to authorized users.
