Spatially controlled cell engineering on biomaterials using polyelectrolytes
Spatially controlled cell engineering on biomaterials using polyelectrolytes
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作者：
Kumar, G;Wang, YC;Ho, CC
控制细胞的空间组织是构建具有血管或神经细胞分布网络的组织的关键一步。在这里，我们报告了一种新的基于软光刻的方法，用于在可生物降解的壳聚糖基质表面上对蛋白质和细胞进行微图案化，这种基质比目前用于细胞微图案化研究的金、银、玻璃或硅基基质更适用于工程组织。在这种方法中，我们使用抗蛋白质和细胞吸附的低聚（乙二醇）甲基丙烯酸酯（OEGMA）和通过酸碱相互作用强烈粘附在壳聚糖底物上的甲基丙烯酸（MA）的随机共聚物，在壳聚糖表面形成稳定的蛋白质和抗细胞微图案。在OEGMA与MA的最佳比例下，形成的共聚物即使在细胞培养条件下也能长期抵抗蛋白质吸附和细胞粘附。利用人微血管内皮细胞，证明了OEGMA/MA微图壳聚糖对细胞组织和排列的空间控制。
Controlling the spatial organization of cells is a critical step toward engineering tissues with distributed networks of blood vessels or nerve cells. Here we report a new soft-lithography-based approach for micropatterning proteins and cells on the surface of biodegradable chitosan substrates that are more applicable to engineering tissues than the gold, silver, glass, or silicone substrates currently used in cell micropatterning studies. In this approach, we use random copolymers of oligo(ethylene glycol) methacrylate (OEGMA), which resists protein and cell adsorption, and methacrylic acid (MA), which adheres strongly onto the chitosan substrate via acid-base interactions, to form stable protein and cell resistant micropatterns on chitosan surfaces. At optimal ratios of OEGMA to MA, copolymers are formed that exhibit superior long-term resistance to protein adsorption and cell adhesion even under cell culture conditions. Spatial control of cell organization and alignment using OEGMA/MA micropatterned chitosan is demonstrated using human microvascular endothelial cells.