Chemical and Transcriptomic Analysis of Cuticle Lipids under Cold Stress in Thellungiella salsuginea
Chemical and Transcriptomic Analysis of Cuticle Lipids under Cold Stress in Thellungiella salsuginea
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DOI：
10.3390/ijms20184519
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发表时间：
2019-09-02
影响因子：
5.6
通讯作者：
Xu, Xiaojing
中科院分区：
文献类型：
--
作者：
He, Junqing;Tang, Shuai;Xu, Xiaojing
植物角质层脂质形成外部保护层以抵抗环境胁迫;然而，角质层性质与耐寒性之间的关系尚不清楚。在这里，极端植物盐芥强调在寒冷的条件下（4摄氏度）和莲座叶的角质层进行了检查的表皮蜡晶体形态，化学成分，角质层相关的基因表达。结果表明，冷诱导角质层超微结构内形成明显的片层。冷胁迫导致单位叶面积总蜡质和角质单体含量分别增加14.58%和12.04%，可能与总脂肪酸含量增加有关。对冷胁迫24 h的小蓟莲座叶进行转录组学分析。我们分析了72个参与角质层脂质代谢的基因的表达，其中一些基因在冷暴露24 h和1周后通过qRT-PCR（定量逆转录PCR）进行了验证。大多数角质层相关基因在低温条件下表现出更高的表达水平，一些关键基因在一周内的表达量比仅仅24 h后的表达量增加得更明显，这可能与角质层中某些成分的含量增加有关。这些结果表明，角质层提供了某些方面的冷适应在T。柳糖
Plant cuticle lipids form outer protective layers to resist environmental stresses; however, the relationship between cuticle properties and cold tolerance is unclear. Here, the extremophyte Thellungiella salsuginea was stressed under cold conditions (4 degrees C) and the cuticle of rosette leaves was examined in terms of epicuticular wax crystal morphology, chemical composition, and cuticle-associated gene expression. The results show that cold induced formation of distinct lamellas within the cuticle ultrastructure. Cold stress caused 14.58% and 12.04% increases in the amount of total waxes and cutin monomer per unit of leaf area, respectively, probably associated with the increase in total fatty acids. The transcriptomic analysis was performed on rosette leaves of Thellungiella exposed to cold for 24 h. We analyzed the expression of 72 genes putatively involved in cuticle lipid metabolism, some of which were validated by qRT-PCR (quantitative reverse transcription PCR) after both 24 h and one week of cold exposure. Most cuticle-associated genes exhibited higher expression levels under cold conditions, and some key genes increased more dramatically over the one week than after just 24 h, which could be associated with increased amounts of some cuticle components. These results demonstrate that the cuticle provides some aspects of cold adaptation in T. salsuginea.