ALLERGEN-INDUCED RECRUITMENT OF INFLAMMATORY CELLS IN LAVAGE 3 AND 24 H AFTER CHALLENGE IN ALLERGIC ASTHMATIC LUNGS
ALLERGEN-INDUCED RECRUITMENT OF INFLAMMATORY CELLS IN LAVAGE 3 AND 24 H AFTER CHALLENGE IN ALLERGIC ASTHMATIC LUNGS
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DOI：
10.1378/chest.103.4.1178
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发表时间：
1993-04-01
期刊：
影响因子：
9.6
通讯作者：
DEMONCHY, JGR
中科院分区：
文献类型：
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作者：
AALBERS, R;KAUFFMAN, HF;DEMONCHY, JGR
为了确定气道炎症细胞在支气管和支气管肺泡水平上的募集与过敏原激发后过敏原诱导的支气管高反应性增加之间是否存在联系，我们使用支气管灌洗和支气管肺泡灌洗来评估气道对过敏原的反应。研究了12例有症状的特应性哮喘患者。在所有患者进行支气管和支气管肺泡灌洗前，3小时，24小时后过敏原的挑战。使用针对T细胞（CD 3、CD 4、CD 8）和嗜酸性粒细胞阳离子蛋白（EG 2）的单克隆抗体。8例患者表现出双重哮喘反应; 4例患者在过敏原激发后仅表现出早期哮喘反应。支气管和支气管肺泡灌洗之间存在明显差异。活化嗜酸性粒细胞（EG 2）在3 h（p = 0.01）和24 h（p = 0.005）均显著增加。双重应答者的活化嗜酸性粒细胞数量显著较高。在临床上发生迟发性哮喘反应（LAR）的患者中，观察到迟发性哮喘反应（LAR）的严重程度与3 h时支气管恢复的上皮细胞数量之间存在相关性，但与24 B时无关。激发后3和24 h，在支气管和支气管肺泡恢复期均未观察到CD 3、CD 4和CD 8细胞数量的显著变化。我们的结论是，在支气管灌洗液中活化的嗜酸性粒细胞的数量与LAR的发展和过敏原诱导的支气管高反应性增加。
To determine whether a link exists between the recruitment of inflammatory cells in the airways on a bronchial and bronchoalveolar level and the development of allergen-induced increase in bronchial hyperresponsiveness after allergen challenge, we used bronchial lavage and bronchoalveolar lavage to assess the airway responses to allergen. Twelve symptomatic atopic asthmatics were studied. In all patients bronchial and bronchoalveolar lavage was performed before, 3 h, and 24 h after allergen challenge. Monoclonal antibodies were used directed against T cells (CD3, CD4, CD8) and the eosinophil cationic protein (EG2). Eight patients showed a dual asthmatic response; four patients showed only an early asthmatic reaction after allergen challenge. Clear differences were found between bronchial and bronchoalveolar lavage. Activated eosinophils (EG2) were significantly increased both at 3 h (p = 0.01) and 24 h (p = 0.005). The number of activated eosinophils was significantly higher in the dual responders. A correlation was observed between the severity of the late asthmatic reaction (LAR) and the number of epithelial cells in the bronchial recovery at 3 h, but not at 24 b, in patients who clinically developed a LAR. No significant changes in the number of CD3, CD4, and CD8 cells 3 and 24 h after the challenge both in the bronchial and bronchoalveolar recovery were observed. We conclude that the number of activated eosinophils in bronchial lavage is associated with the development of the LAR and allergen-induced increase in bronchial hyperresponsiveness.