Advances in studying whole mouse brain vasculature using high-resolution 3D light microscopy imaging.
Advances in studying whole mouse brain vasculature using high-resolution 3D light microscopy imaging.
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DOI：
10.1117/1.nph.9.2.021902
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发表时间：
2022-04
期刊：
影响因子：
5.3
通讯作者：
Kim Y
中科院分区：
文献类型：
--
作者：
Bennett HC;Kim Y
脑血管系统越来越被认为是整个大脑健康和许多大脑疾病的主要参与者。尽管已经有几项具有里程碑意义的研究在解剖学定义的区域内了解这些至关重要的结构的细节，但对整个脑血管系统（包括微血管）的全脑检查一直具有挑战性。然而，新兴技术，包括组织处理和三维（3D）显微镜成像，使神经科学家能够检查整个小鼠大脑中的总脉管系统。在这里，我们的目标是突出这些高分辨率的3D映射方法的进展，包括块面成像和光片荧光显微镜。我们总结了最新的制图工具，以了解脑血管网络的详细解剖安排和神经血管单元（NVU）作为一个整体的组织原则。我们讨论了从使用这些成像方法的研究中获得的生物学见解，以及如何使用这些工具来推进我们对整个大脑中脑血管网络和相关细胞类型的理解。本文将有助于了解高分辨率小鼠NVU定位的最新进展，并为今后的研究提供展望。
The cerebrovasculature has become increasingly recognized as a major player in overall brain health and many brain disorders. Although there have been several landmark studies to understand details of these crucially important structures in an anatomically defined area, brain-wide examination of the whole cerebrovasculature, including microvessels, has been challenging. However, emerging techniques, including tissue processing and three-dimensional (3D) microscopy imaging, enable neuroscientists to examine the total vasculature in the entire mouse brain. Here, we aim to highlight advances in these high-resolution 3D mapping methods including block-face imaging and light sheet fluorescent microscopy. We summarize latest mapping tools to understand detailed anatomical arrangement of the cerebrovascular network and the organizing principles of the neurovascular unit (NVU) as a whole. We discuss biological insights gained from studies using these imaging methods and how these tools can be used to advance our understanding of the cerebrovascular network and related cell types in the entire brain. This review article will help to understand recent advance in high-resolution NVU mapping in mice and provide perspective on future studies.