Determination of Free Fatty Acids in Human Bile by High-Performance Liquid Chromatography
Determination of Free Fatty Acids in Human Bile by High-Performance Liquid Chromatography
复制标题
高效液相色谱法测定人胆汁中的游离脂肪酸
DOI：
10.1177/000456329803500213
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发表时间：
1998
影响因子：
2.2
通讯作者：
K. Ogoshi
中科院分区：
文献类型：
--
作者：
Y. Hori;Kazutoshi Nakamura;Masaharu Yamamoto;K. Shimada;H. Nakadaira;N. Shibuya;K. Endoh;K. Ogoshi
建立了胆汁中游离脂肪酸的高效液相色谱分析方法。在该方法中，使用Isolute™ ODS柱（用9-蒽基重氮甲烷（ADAM）衍生），在一个步骤中从胆汁中提取FFA。选择ADAM是因为其在室温下与羧酸的高反应性。然后，使用HPLC分离和定量FFA。这种方法被证明是简单和节省时间。游离脂肪酸的平均回收率为97.6%，检测限为100-250 pg。用此方法测定了11例胆囊结石患者胆囊胆汁中游离脂肪酸的浓度。总游离脂肪酸的平均浓度为0.61（SD = 0.41）mmol/L，个体游离脂肪酸的变异性很大。
We developed a high-performance liquid chromatography (HPLC) method for free fatty acids (FFAs) analysis in bile. In this method, FFAs were extracted from bile in a single step using an Isolute™ ODS cartridge, derivatized with 9-anthryldiazomethane (ADAM). ADAM was chosen because of its high reactivity with carboxylic acid at room temperature. Then, HPLC was used for separating and quantifying FFAs. This method proved to be simple and time-saving. The mean recovery of FFA added to human gallbladder bile was 97.6%, and the detection limit was 100–250 pg. Using this method, we determined FFA concentrations in the gallbladder bile of 11 gallstone patients. The mean concentration of total FFA was 0.61 (SD = 0.41) mmol/L, and there was wide variation in the individual FFAs.