Free secretory component as a standardization protein for nasopharyngeal specimens from children with upper respiratory tract infection.
Free secretory component as a standardization protein for nasopharyngeal specimens from children with upper respiratory tract infection.
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游离分泌成分作为上呼吸道感染儿童鼻咽标本的标准化蛋白。
DOI：
10.1080/08035259950170303
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Chonmaitree,T
中科院分区：
文献类型：
--
作者：
Heikkinen,T;Shenoy,M;Goldblum,RM;Chonmaitree,T
游离分泌成分（FSC）已被推荐为一种可靠的蛋白质，用于校正早产儿气管吸出物样本中的未知稀释度。为了研究FSC是否也能为鼻咽分泌物样本提供有效的标准化蛋白质，本研究确定了35名儿童（中位年龄14个月）鼻分泌物中FSC浓度随时间的变化和变化，这些儿童参加了抗生素疗效试验。在入组时和2-3天后获得鼻咽抽吸物。通过直接酶免疫测定法定量标本中的FSC。鼻分泌物中FSC的浓度范围为0.08 - 189.6 μgml−1（中位数12.3 μgml−1）;最高浓度与最低浓度的比值为2370，第90百分位数与第10百分位数浓度之间的差异为189倍，第75百分位数与第25百分位数值之间的差异为26。年龄<12个月的儿童FSC浓度（中位数2.2 μg ml−1）显著低于年龄较大的儿童（中位数21.5 μg ml−1;p= 0.035）。在第一次和随访标本之间，65%的儿童在分泌物中的FSC水平差异>2倍。由于最佳标准化蛋白质应显示个体之间和随时间的最小变化，FSC可能不是校正上呼吸道感染儿童鼻咽标本未知稀释度的合适蛋白质。
Free secretory component (FSC) has been recommended as a reliable protein for correction of the unknown dilution in tracheal aspirate samples from preterm infants. To investigate whether FSC would also provide a valid standardization protein for samples of nasopharyngeal secretions, this study determined the intersubject variation and the alteration over time in the concentrations of FSC in nasal secretions from 35 children (median age 14 months) who participated in an antibiotic efficacy trial. Nasopharyngeal aspirates were obtained at enrolment and after 2–3 d. FSC in the specimens was quantified by a direct enzyme immunoassay. The concentrations of FSC in the nasal secretions ranged from 0.08 to 189.6 μgml−1(median 12.3 μgml−1); the ratio of the highest to the lowest concentrations was 2370, the difference between the 90th and 10th percentile concentrations was 189‐fold and the difference between the 75th and 25th percentile values was 26. FSC concentrations were significantly lower in children aged <12 months (median 2.2 μg ml−1) than in the older children (median 21.5 μg ml−1;p= 0.035). Between the first and the follow‐up specimens, 65% of the children had >2‐fold difference in the levels of FSC in the secretions. Because an optimal standardization protein should show minimal variation between individuals and over time, FSC may not be a suitable protein for correction of the unknown dilution of nasopharyngeal specimens from children with upper respiratory tract infection.