Two novel alkalitolerant species Pseudalkalibacillus spartinae sp. nov. and Pseudalkalibacillus sedimenti sp. nov.
Two novel alkalitolerant species Pseudalkalibacillus spartinae sp. nov. and Pseudalkalibacillus sedimenti sp. nov.
复制标题
两个新的耐碱菌种 Pseudalkalibacillus spartinae sp.
DOI：
10.1099/ijsem.0.006159
复制
发表时间：
2023
影响因子：
2.8
通讯作者：
Shunhua Zhou
中科院分区：
文献类型：
--
作者：
Guo;Shuang Han;Bing Li;Rui;Huai Shi;Qianqian Chen;H. Alwathnani;Christopher Rensing;Shunhua Zhou
在本研究中，从中国漳州收集的沉积物样品中分离出两种新型耐碱菌株（FJAT-53046T 和 FJAT-53715T）。基于16S rRNA基因序列的系统发育表明菌株FJAT-53046T和FJAT-53715T是Pseudalkalibacillus属的新成员。两个新菌株与 Pseudalkalibacillus hwajinpoensis DSM 16206T 的 16S rRNA 基因序列相似性最高，分别为 97.4% 和 97.6%。两个菌株与参考菌株之间的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值分别为77.2和79.6%、20.9和30.2%，低于原核物种划分阈值。菌株FJAT-53046T和FJAT-53715T之间的ANI和dDDH值分别为86.0和30.2 %，表明它们属于Pseudalkalibacillus属的不同种。两种菌株的主要呼吸醌为MK-7，主要的细胞脂肪酸为反异-C15 : 0和反异-C17 : 0。二磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺是两种新菌株的主要极性脂质。结合物理和生化特征、化学性质和基因组分析的测定结果，菌株 FJAT-53046T 和 FJAT-53715T 被认为代表 Pseudalkalibacillus 属的两个新物种，其名称为 Pseudalkalibacillus spartinae sp.。十一月和沉积假碱杆菌。十一月被提议。模式菌株分别是FJAT-53046T(＝GDMCC 1.3077T＝JCM 35611T)和FJAT-53715T(＝GDMCC 1.3076T＝JCM 35610T)。
In this study, two novel alkalitolerant strains (FJAT-53046T and FJAT-53715T) were isolated from sediment samples collected in Zhangzhou, PR China. Phylogeny based on 16S rRNA gene sequences suggested that strains FJAT-53046T and FJAT-53715T were new members of the genus Pseudalkalibacillus. The two novel strains showed the highest 16S rRNA gene sequence similarity to Pseudalkalibacillus hwajinpoensis DSM 16206T, with values of 97.4 and 97.6 %, respectively. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the two strains and the reference strain were 77.2 and 79.6 %, 20.9 and 30.2 %, respectively, which were below the prokaryotic species delineation thresholds. The ANI and dDDH values between strains FJAT-53046T and FJAT-53715T were 86.0 and 30.2 %, respectively, suggesting that they belonged to different species in the genus Pseudalkalibacillus. The major respiratory quinone in both strains was MK-7 and the major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the major polar lipids in both novel strains. Combined with results stemming from the determination of physical and biochemical characteristics, chemical properties, and genome analysis, strains FJAT-53046T and FJAT-53715T are proposed to represent two novel species of the genus Pseudalkalibacillus, for which the names Pseudalkalibacillus spartinae sp. nov. and Pseudalkalibacillus sedimenti sp. nov. are proposed. The type strains are FJAT-53046T (=GDMCC 1.3077T=JCM 35611T) and FJAT-53715T (=GDMCC 1.3076T=JCM 35610T), respectively.
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro