Comparative Analysis of USA300 Virulence Determinants in a Rabbit Model of Skin and Soft Tissue Infection
Comparative Analysis of USA300 Virulence Determinants in a Rabbit Model of Skin and Soft Tissue Infection
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DOI：
10.1093/infdis/jir441
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发表时间：
2011-09-15
影响因子：
6.4
通讯作者：
DeLeo, Frank R.
中科院分区：
文献类型：
--
作者：
Kobayashi, Scott D.;Malachowa, Natalia;DeLeo, Frank R.
社区相关耐甲氧西林金黄色葡萄球菌（CA-MRSA）感染通常与携带编码Panton-Valentine杀白细胞素（PVL）基因的菌株相关。PVL在流行性CA-MRSA菌株USA 300成功中的作用仍然未知。在这里，我们开发了一个皮肤和软组织感染模型，在兔子测试的假设，PVL有助于USA 300的发病机制，并比较它与公认的毒力决定因素：α-溶血素（Hla），酚可溶性调制蛋白-α肽（PSM α），和辅助基因调节（Agr）。这些数据表明，Hla，PSM α，和Agr的USA 300皮肤感染的发病机制在兔，而PVL的作用不能检测。
Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are frequently associated with strains harboring genes encoding Panton-Valentine leukocidin (PVL). The role of PVL in the success of the epidemic CA-MRSA strain USA300 remains unknown. Here we developed a skin and soft tissue infection model in rabbits to test the hypothesis that PVL contributes to USA300 pathogenesis and compare it with well-established virulence determinants: alpha-hemolysin (Hla), phenol-soluble modulin-alpha peptides (PSM alpha), and accessory gene regulator (Agr). The data indicate that Hla, PSM alpha, and Agr contribute to the pathogenesis of USA300 skin infections in rabbits, whereas a role for PVL could not be detected.