Extended-spectrum-β-lactamase- and AmpC-β-lactamase-producing Escherichia coli in Dutch broilers and broiler farmers
Extended-spectrum-β-lactamase- and AmpC-β-lactamase-producing Escherichia coli in Dutch broilers and broiler farmers
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DOI：
10.1093/jac/dks349
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发表时间：
2013-01-01
影响因子：
5.2
通讯作者：
Mevius, Dik
中科院分区：
文献类型：
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作者：
Dierikx, Cindy;van der Goot, Jeanet;Mevius, Dik
本研究的目的是确定荷兰肉鸡养殖场和农民中产超广谱内酰胺酶（ESBL）和AmpC -内酰胺酶大肠杆菌的流行率，并比较农民及其动物中产ESBL/AmpC的分离株。从26个鸡场的每一个鸡场采集的5至41个泄殖腔拭子和从18个肉鸡养殖户采集的18个粪便样品进行了分析，以确定是否存在产ESBL/AmpC的E.杆菌ESBL/AmpC基因的鉴定采用基因芯片、PCR和测序技术。质粒通过转化和基于PCR的复制子分型来表征。质粒分型采用质粒多位点序列分型或限制性片段长度多态性。E.用多位点序列分型法对鸡群产ESBL/AmpC大肠杆菌进行基因分型，结果表明，所有鸡群均为产ESBL/AmpC大肠杆菌阳性。26个农场中有22个农场的农场内患病率为1.80。18名农民中有6名携带ESBL/AmpC基因bla（CTX-M-1）、bla（CMY-2）和/或bla（SHV-12）的分离株，这些分离株也存在于他们的动物样品中。其中5个分离株的基因位于相同的质粒家族[IncI 1（n3）、IncK（n1）或IncN（n1）]上，而来自2个农民的分离株的基因携带在与来自其动物的分离株相同的质粒亚型上（IncI 1 ST 12和IncN ST 1，其中ST代表序列类型）。大肠杆菌和产ESBL/AmpC大肠杆菌的高流行率。农民的大肠杆菌。这是不期望的，因为这对人类健康构成风险。未来的研究应侧重于鉴定肉鸡生产链中这些分离株的来源，以使减少这些分离株的干预措施成为可能。
The aim of this study was to establish the prevalence of extended-spectrum -lactamase (ESBL)- and AmpC -lactamase-producing Escherichia coli at Dutch broiler farms and in farmers and to compare ESBL/AmpC-producing isolates from farmers and their animals.Twenty-five to 41 cloacal swabs collected from broilers at each of 26 farms and 18 faecal samples from 18 broiler farmers were analysed for determination of the presence of ESBL/AmpC-producing E. coli. ESBL/AmpC genes were characterized by microarray, PCR and sequencing. Plasmids were characterized by transformation and PCR-based replicon typing. Subtyping of plasmids was done by plasmid multilocus sequence typing or restriction fragment length polymorphism. E. coli genotypes were determined by multilocus sequence typing.Birds from all farms were positive for ESBL/AmpC-producing E. coli, and on 22/26 farms the within-farm prevalence was epsilon 80. Six of 18 farmers carried isolates containing ESBL/AmpC genes bla(CTX-M-1), bla(CMY-2) and/or bla(SHV-12), which were also present in the samples from their animals. In five of these isolates, the genes were located on identical plasmid families [IncI1 (n3), IncK (n1) or IncN (n1)], and in isolates from two farmers the genes were carried on identical plasmid subtypes (IncI1 ST12 and IncN ST1, where ST stands for sequence type) as in the isolates from their animals.This study shows a high prevalence of birds carrying ESBL/AmpC-producing E. coli at Dutch broiler farms and a high prevalence of ESBL/AmpC-producing E. coli in farmers. This is undesirable due to the risk this poses to human health. Future research should focus on identification of the source of these isolates in the broiler production chain to make interventions resulting in reduction of these isolates possible.