Applications of Ultrafiltration-Nanofiltration Membrane Continuous Combination Technology for Refining of Milk-Derived Oligosaccharides
Applications of Ultrafiltration-Nanofiltration Membrane Continuous Combination Technology for Refining of Milk-Derived Oligosaccharides
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DOI：
10.4028/www.scientific.net/amr.634-638.1429
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发表时间：
2013-01
期刊：
影响因子：
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通讯作者：
S. An;Jinhong Wu;L. Qian;Yanhong Gao;Yan Wu;Guoping Yu
中科院分区：
文献类型：
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作者：
S. An;Jinhong Wu;L. Qian;Yanhong Gao;Yan Wu;Guoping Yu
采用保加利亚乳酸菌发酵生产低聚半乳糖（GOS），并采用本文建立的超滤-纳滤连续膜组合工艺对GOS进行进一步精制。结果表明，当料液浓度为2.5g/L时，超滤的最佳操作条件为0.05MPa，纳滤的最佳操作条件为45 °C、0.4MPa。采用膜组合技术精制后，蛋白质去除率达96.70%，精制产品的相对质量比提高22倍左右，主要含有DP 3和DP 2的低聚物，主要分布在108 ~ 2415 Da之间，表明超滤-纳滤连续膜组合技术是一种新的、有利的浓缩或精制发酵乳中GOS的工艺方法。
The galactooligosaccharide (GOS) was produced by Bulgarian lactic acid bacteria fermentation and further refined by application of an ultrafiltration-nanofiltration continuous membrane combination technology established in this paper. Results revealed that the optimum of membrane operation conditions for ultrafiltration were 0.05 Mpa at the feed concentration of 2.5 g/L, and the optimal conditions for nanofiltration were 45 °C, 0.4 Mpa. After refining by membrane combination technology, 96.70% of proteins were removed and the relative quality ratio of the refined GOS product was increased by 22 times or so, which mainly contained oligomers with DP 3 and DP 2, and mostly distributed between 108 Da and 2415 Da. These findings suggest that the ultrafiltration-nanofiltration continuous membrane combination technology is a new and favorable processes method for enrich or refining of milk-derived GOS obtained by fermentation.