Multipolar Migration: The Third Mode of Radial Neuronal Migration in the Developing Cerebral Cortex
Multipolar Migration: The Third Mode of Radial Neuronal Migration in the Developing Cerebral Cortex
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DOI：
10.1523/jneurosci.23-31-09996.2003
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发表时间：
2003-11
期刊：
影响因子：
--
通讯作者：
H. Tabata;K. Nakajima
中科院分区：
文献类型：
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作者：
H. Tabata;K. Nakajima
在发育中的大脑皮层中，有两种不同的放射状神经元迁移模式，运动和染色体易位。虽然这两种迁移模式已被很好地记录，皮质中间区含有丰富的多极细胞，它们不像通过运动或染色体易位迁移的细胞。在这里，我们报告说，这些多极细胞表达神经元标记物，并独立于放射状胶质纤维在各个方向延伸多个薄的过程。对活体切片的延时分析显示，多极细胞没有任何固定的细胞极性，随着细胞体的缓慢移动，它们非常动态地伸展和收缩多个突起。它们在径向迁移过程中通常不会直接向软脑膜表面移动，而是经常改变迁移方向和速率;有时它们甚至保持在几乎相同的位置，特别是当它们位于脑室下区时。有时，多极细胞在其径向迁移过程中切向跳跃。由于这些细胞的迁移方式明显不同于运动或染色体易位，我们将其新型迁移称为“多极迁移”。鉴于呈现多极迁移的细胞比例很高，这第三种放射状迁移模式一定是发育中皮质的一种重要迁移类型。
Two distinct modes of radial neuronal migration, locomotion and somal translocation, have been reported in the developing cerebral cortex. Although these two modes of migration have been well documented, the cortical intermediate zone contains abundant multipolar cells, and they do not resemble the cells migrating by locomotion or somal translocation. Here, we report that these multipolar cells express neuronal markers and extend multiple thin processes in various directions independently of the radial glial fibers. Time-lapse analysis of living slices revealed that the multipolar cells do not have any fixed cell polarity, and that they very dynamically extend and retract multiple processes as their cell bodies slowly move. They do not usually move straight toward the pial surface during their radial migration, but instead frequently change migration direction and rate; sometimes they even remain in almost the same position, especially when they are in the subventricular zone. Occasionally, the multipolar cells jump tangentially during their radial migration. Because the migration modality of these cells clearly differs from locomotion or somal translocation, we refer to their novel type of migration as “multipolar migration.” In view of the high proportion of cells exhibiting multipolar migration, this third mode of radial migration must be an important type of migration in the developing cortex.