Fluorescent nanoprobe for in-vivo ratiometric imaging of endogenous hydrogen peroxide resulted from drug-induced organ damages
Fluorescent nanoprobe for in-vivo ratiometric imaging of endogenous hydrogen peroxide resulted from drug-induced organ damages
复制标题
用于药物引起的器官损伤引起的内源性过氧化氢体内比例成像的荧光纳米探针
DOI：
10.1016/j.bios.2017.03.003
复制
发表时间：
2017-08-15
影响因子：
12.6
通讯作者：
Wu, Shuizhu
中科院分区：
文献类型：
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作者：
Peng, Jin;Hou, Xianfeng;Wu, Shuizhu
药物性器官损害已成为严重的公共卫生问题，因此有效的药物性器官损害的体内检测方法具有重要意义。本研究将纳米荧光探针分子负载到磷脂双分子层中，制备了一种用于检测体内过氧化氢（H2 O2，一种器官损伤生物标志物）水平的比率荧光纳米探针（NPs-A）。该探针分子的电子物理行为取决于蒽环6位基团的吸电子能力，在蒽环6位上引入了过氧化氢识别部分（二羰基与硝基苯基偶联，简称硝基苯基-二羰基）。探针与H2 O2反应后，硝基苯基二羰基转化为羧基，由于第6位取代基吸电子能力的变化，探针分子的荧光性质也随之变化，从而保证了对H2 O2的高选择性比率检测，检测限为0.49 μ M。此外，纳米探针（NPs-A）被应用于细胞和体内成像应用;结果表明，它可以检测和跟踪活细胞中的H2 O2水平，并监测和空间映射内源性H2 O2水平在药物诱导的斑马鱼器官损伤模型。
Drug-induced organ damages have been considered as a grave problem regarding public health; hence effective method for in vivo detection of drug-induced organ damages is of great significance. Herein we developed a ratiometric fluorescent nanoprobe (NPs-A), which was prepared by loading the probe molecules into phospholipid bilayer, for assaying hydrogen peroxide (H2O2, an organ damage biomarker) level in vivo. The photophysical behavior of the probe molecule depends on the electron-withdrawing ability of the group at the 6 position of anthracene ring, on which the recognition moiety for hydrogen peroxide (dicarbonyl coupled with nitrophenyl, referred to as nitrophenyl-dicarbonyl) was introduced. Upon the reaction of the probe with H2O2, nitrophenyl-dicarbonyl group transforms into carboxyl group, and due to the variation of the electron withdrawing ability of the 6th substituent, the fluorescent properties of the probe molecule alters accordingly, thus ensuring the ratiometric detection for H2O2 with high selectivity with the detection limit of 0.49 mu M. In addition, the nanoprobe (NPs-A) was applied for cell and in vivo imaging applications; and the results indicate that it can detect and track the level of H2O2 in living cells and to monitor and spatially map endogenous H2O2 levels in a drug-induced organ damage model of zebrafish.