Fourier Transform Infrared Spectroscopic Imaging of Fracture Healing in the Normal Mouse
Fourier Transform Infrared Spectroscopic Imaging of Fracture Healing in the Normal Mouse
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DOI：
10.1155/2015/659473
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发表时间：
2015-01-01
影响因子：
2
通讯作者：
Boskey, Adele L.
中科院分区：
文献类型：
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作者：
Gollwitzer, Hans;Yang, Xu;Boskey, Adele L.
傅里叶变换红外光谱成像（FTIRI）用于通过对野生型小鼠的各种愈伤组织进行空间分析来研究骨愈合。通过截骨术使 28 只雄性 C57BL 小鼠发生股骨骨折。在第 1、2、4 和 8 周处死动物以获得处于明确愈合阶段的愈伤组织。微计算机断层扫描后，将骨样本切成连续切片进行 FTIRI 和组织学分析，从而实现两种成像方法在不同愈伤组织区域（早期钙化软骨、编织骨、膜内和软骨内骨形成区域）的空间相关性。根据 FTIRI，所有骨痂区域的矿物质/基质比率在骨折愈合的前 4 周内显着增加，并且与微型 CT 测量的骨矿物质密度相关。新形成的钙化组织中碳酸盐/磷酸盐比率升高，并在第 2 周达到与皮质骨相当的值。第 1-8 周期间，大多数组织中的胶原成熟度和矿物质结晶度增加，而酸式磷酸盐替代量减少。在整个愈合期间检测到颞部和愈伤组织面积依赖性变化。这些数据证实了 FTIRI 在评估小鼠骨折愈合方面的有用性及其评估病理性骨折愈合和治疗干预效果的潜力。
Fourier transform infrared spectroscopic imaging (FTIRI) was used to study bone healing with spatial analysis of various callus tissues in wild type mice. Femoral fractures were produced in 28 male C57BL mice by osteotomy. Animals were sacrificed at 1, 2, 4, and 8 weeks to obtain callus tissue at well-defined healing stages. Following microcomputerized tomography, bone samples were cut in consecutive sections for FTIRI and histology, allowing for spatial correlation of both imaging methods in different callus areas (early calcified cartilage, woven bone, areas of intramembranous and endochondral bone formation). Based on FTIRI, mineral/matrix ratio increased significantly during the first 4 weeks of fracture healing in all callus areas and correlated with bone mineral density measured by micro-CT. Carbonate/phosphate ratio was elevated in newly formed calcified tissue and at week 2 attained values comparable to cortical bone. Collagen maturity and mineral crystallinity increased during weeks 1-8 inmost tissues while acid phosphate substitution decreased. Temporal and callus area dependent changes were detected throughout the healing period. These data assert the usefulness of FTIRI for evaluation of fracture healing in the mouse and its potential to evaluate pathologic fracture healing and the effects of therapeutic interventions.