Detection of fetal Rhesus D and sex using fetal DNA from maternal plasma by multiplex polymerase chain reaction
Detection of fetal Rhesus D and sex using fetal DNA from maternal plasma by multiplex polymerase chain reaction
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DOI：
10.1111/j.1471-0528.2000.tb13338.x
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发表时间：
2000-06-01
期刊：
影响因子：
--
通讯作者：
Hahn, S
中科院分区：
文献类型：
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作者：
Zhong, XY;Holzgreve, W;Hahn, S
目的应用聚合酶链反应法从孕妇血浆中提取胎儿DNA，检测胎儿性别和恒河猴D基因型的敏感性、特异性和可重复性。方法对22例拟行有创手术的D型恒河猴阴性孕妇进行血液采集。从血浆部分提取DNA，并使用Y染色体和恒河猴d特异性引物进行多重巢式聚合酶链反应分析。该实验程序的结果与通过侵入性程序获得的材料的分析结果进行了比较。结果基于血浆聚合酶链反应的方法的敏感性惊人地高，在几乎100%的检查病例中正确确定了两种胎儿基因型。只有一例记录了D型恒河猴检测的假阳性结果，随后的分析结果为阴性。结论基于血浆聚合酶链反应的方法简便、快速，是一种很有前景的多胎儿基因位点分析方法，如恒河猴、性别等。
Objective To test the sensitivity, specificity and reproducibility using fetal DNA obtained from plasma of pregnant women by polymerase chain reaction for the simultaneous detection of both fetal sex and Rhesus D genotype.Method Blood samples were obtained from 22 Rhesus D negative pregnant women about to undergo an invasive procedure. DNA was extracted from the plasma fraction and analysed by a multiplex nested polymerase chain reaction using Y chromosome-and Rhesus D-specific primers. The results of this experimental procedure were compared with those obtained from the analysis performed on material gained by the invasive procedure.Results The sensitivity of the plasma polymerase chain reaction-based method was surprisingly high, with both fetal genotypes being correctly determined in almost 100% of the cases examined. In only one instance was a false positive result for the detection of Rhesus D recorded, which on subsequent analysis was negative.Conclusions The ease and rapidity with which the plasma polymerase chain reaction-based method can be performed makes it a promising method for the analysis of multiple fetal loci, such as Rhesus D and sex.