Mapping of domains on the human parainfluenza virus type 2 nucleocapsid protein (NP) required for NP-phosphoprotein or NP-NP interaction
Mapping of domains on the human parainfluenza virus type 2 nucleocapsid protein (NP) required for NP-phosphoprotein or NP-NP interaction
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DOI：
10.1099/0022-1317-80-8-2017
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发表时间：
1999-08-01
影响因子：
3.8
通讯作者：
Ito, Y
中科院分区：
文献类型：
--
作者：
Nishio, M;Tsurudome, M;Ito, Y
通过测试41种抗人副流感病毒2型（hPIV-2）核壳蛋白（NP）的单克隆抗体与缺失突变体的反应性，将其识别的表位定位在hPIV-2 NP蛋白的一级结构上，通过使用这些单克隆抗体的Western免疫印迹，对hPIV-2NP蛋白的缺失突变体进行分析，以鉴定NP蛋白上NP-NP相互作用所必需的区域和磷蛋白（P）结合位点。结果表明，NP蛋白N端294个氨基酸是NP-NP自组装所必需的，NP蛋白C端的两个氨基酸是NP-P结合所必需的：一个氨基酸295-402是与P蛋白C端结合所必需的，另一个氨基酸403-494是与P蛋白N端结合所必需的。
The epitopes recognized by 41 monoclonal antibodies directed against the nucleocapsid protein (NP) of human parainfluenza virus type 2 (hPIV-2) were mapped on the primary structure of the hPIV-2 NP protein by testing their reactivities with deletion mutants, By Western immunoblotting using these monoclonal antibodies, the analysis of deletion mutants of the hPIV-2 NP protein was performed to identify the region essential for NP-NP interaction and phosphoprotein (P)-binding sites on the NP protein. The results indicate that the N-terminal 294 aa of the NP protein are all required for NP-NP self-assembly, and that two C-terminal parts of the NP protein are essential for NP-P binding: one region, aa 295-402, is required for binding to the C-terminal part of the P protein and another region, aa 403-494, to the N-terminal part of the P protein.