A solution for universal classification of species based on genomic DNA.
A solution for universal classification of species based on genomic DNA.
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基于基因组DNA的物种普遍分类的解决方案。
DOI：
10.1155/2007/27894
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
Nishigaki, Koichi
中科院分区：
文献类型：
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作者：
Kouduka, Mariko;Sato, Daisuke;Komori, Manabu;Kikuchi, Motohiro;Miyamoto, Kiyoshi;Kosaku, Akinori;Naimuddin, Mohammed;Matsuoka, Atsushi;Nishigaki, Koichi
传统上，生物体是根据其表型进行分类的。最近，通过测序技术的发展，基于基因型的分类已经成为可能。然而，由于成本和劳动力，将测序方法应用于大量物种的分析仍然是困难的。在大多数生物学领域中，分析由各种物种组成的复杂系统已成为一个重要的主题，需要一种有效的方法来处理大量的物种。在本文中，我们已经证明，使用植物，鱼类和昆虫，基因组分析，一个紧凑的基因组分析技术，可以普遍分类生物。令人惊讶的是，在所有三个领域的生物测试，从表型生成的系统发育树拓扑完全匹配的基因型生成的。此外，单个探针足以用于基因组谱分析，从而证明该方法是通用和紧凑的。
Traditionally, organisms have been classified on the basis of their phenotype. Recently, genotype-based classification has become possible through the development of sequencing technology. However, it is still difficult to apply sequencing approaches to the analysis of a large number of species due to the cost and labor. In most biological fields, the analysis of complex systems comprising various species has become an important theme, demanding an effective method for handling a vast number of species. In this paper, we have demonstrated, using plants, fish, and insects, that genome profiling, a compact technology for genome analysis, can classify organisms universally. Surprisingly, in all three of the domains of organisms tested, the phylogenetic trees generated from the phenotype topologically matched completely those generated from the genotype. Furthermore, a single probe was sufficient for the genome profiling, thereby demonstrating that this methodology is universal and compact.