Effects of landscape and demographic history on genetic variation in Picea glehnii at the regional scale
Effects of landscape and demographic history on genetic variation in Picea glehnii at the regional scale
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DOI：
10.1007/s11284-009-0611-8
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发表时间：
2009-11-01
影响因子：
2
通讯作者：
Ide, Yuji
中科院分区：
文献类型：
--
作者：
Goto, Susumu;Tsuda, Yoshiaki;Ide, Yuji
为了在区域尺度上评估景观和种群历史对库页云杉（Picea glehnii）遗传变异的影响，我们利用7个简单序列重复（SSR）标记，对日本北海道中部富良野地区的库页云杉种群的遗传多样性和遗传分化进行了研究。我们发现海拔与遗传多样性参数之间存在显著的相关性。A值（[46]）随着海拔的升高而增加，F（IS）值则降低，而所有种群的F（IS）值与0均无显著差异。在低海拔地区的孤立种群以及中海拔和高海拔地区相对较大的种群中检测到近期存在显著的瓶颈效应。应考虑全新世之前的进化事件，因为海拔梯度应与开花物候等本地适应性状相关。然而，该地区全新世的孢粉学数据表明，此处检测到的库页云杉遗传多样性分布模式可能受到过去种群历史的影响，这种历史与该时期气候变化相关的该物种分布的海拔变化有关。种群分化程度较低，F（ST）和G'（ST）值分别为0.022和0.065。然而，在一个沼泽种群（C13）周围检测到了遗传边界。因此，当考虑所有种群时，未检测到显著的距离隔离（IBD），但当排除C13种群时，则存在显著的IBD。区域尺度上的遗传多样性和遗传分化信息可能对库页云杉造林计划的种子源选择有用。
To evaluate the effects of landscape and demographic history on genetic variation in Picea glehnii at a regional scale we have investigated the genetic diversity and genetic differentiation of P. glehnii populations in the Furano region, central Hokkaido, Japan, using seven simple sequence repeat (SSR) markers. We found significant correlations between elevation and genetic diversity parameters. The value of A ([46]) increased and the value of F (IS) decreased with increasing elevation, while F (IS) values were not significantly different from 0 in any of the populations. Significant recent bottlenecks were detected for isolated populations at low-elevation sites and for relatively large populations at moderate- and high-elevation sites. Evolutionary events pre-dating the Holocene should be taken into consideration, as elevational gradients should be with respect to locally adapted traits such as flowering phenology, However, the palynological data from the Holocene in this region suggest that the distribution pattern of genetic diversity of P. glehnii detected here may have been influenced by past demographic history related to the elevation shifts in this species' distribution associated with climate change during this period. Population differentiation was low, with F (ST) and G'(ST) values of 0.022 and 0.065, respectively. However, genetic boundaries were detected around one swamp population (C13). Therefore, significant isolation by distance (IBD) was not detected when all populations were considered, but there was significant IBD when the C13 population was excluded. Information on genetic diversity and genetic differentiation at the regional scale may be useful for selecting seed sources for afforestation programs for P. glehnii.