A genome-wide survey of NOD-like receptors in Chinese tongue sole (Cynoglossus semilaevis): Identification, characterization and expression analysis in response to bacterial infection
A genome-wide survey of NOD-like receptors in Chinese tongue sole (Cynoglossus semilaevis): Identification, characterization and expression analysis in response to bacterial infection
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DOI：
10.1111/jfb.14871
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发表时间：
2021-10-28
影响因子：
2
通讯作者：
Chen, Songlin
中科院分区：
文献类型：
--
作者：
Chen, Zhangfan;Xu, Xiwen;Chen, Songlin
富含亮氨酸重复序列的受体（NLR，NOD-like receptors）作为细胞内病原体识别受体（PRRs），参与脊椎动物的先天性免疫应答。然而，目前对我国常见的海水养殖鱼类半滑舌鳎（Cynoglossus semilaevis）的NLR研究尚未见系统报道。在本研究中，NLR的全基因组调查进行了C。半滑舌鳎的NLR基因，共鉴定出29个NLR基因，包括5个NLR-A亚家族基因（CsNOD 1 -5）、2个NLR-B亚家族基因、18个NLR-C亚家族基因（CsNLR-C1 - 18）和4个其它NLR基因。系统进化分析表明，CsNOD 1 -5与人NOD 1 -5具有同源性，且含有保守的功能结构域。此外，CsNLR-C基因都具有FISHNA结构域，这是一个鱼类特异性的NACHT亚结构域。表达分析表明，CsNOD 1 -5和CsNLR-C1/2在各种正常组织中普遍表达。用哈维氏弧菌感染的细菌揭示了所有测试的CsNLR在鳃、肠、肾、肝和脾中的不同表达模式。特别是，CsNOD 1 -4和CsNLR-C2在细菌感染后48 h在鳃中显著上调。此外，CsNOD 3和CsNOD 4在感染肠、肾、肝和脾中的表达均显著升高，表明其对细菌感染的敏感性高于其他CsNLR。结合CsNLR的蛋白质-蛋白质相互作用网络的计算结果，提示单个NLR基因在C. semilaevi对细菌感染。本研究为进一步研究CsNLR的免疫功能提供了有价值的信息。
As intracellular pathogen recognition receptors (PRRs), nucleotide-binding domain, leucine-rich repeat containing receptors (NLRs, NOD-like receptors) are involved in innate immune responses in vertebrates. However, there is no systemic study on NLRs in Chinese tongue sole (Cynoglossus semilaevis), a popular maricultured fish in China. In the present study, a genome-wide survey of NLRs was performed in C. semilaevis, with the identification of 29 NLRs, including five genes from the NLR-A subfamily (referred to as CsNOD1-5), two genes from the NLR-B subfamily, 18 genes from the NLR-C subfamily (referred to as CsNLR-C1 to 18) and four other NLR genes. Phylogenetic analysis implied that CsNOD1-5 contained conserved functional domains and had orthologous relationships with human NOD1-5. Moreover, CsNLR-C genes all possessed the FISHNA domain, which is a fish-specific NACHT subdomain. Expression analysis showed that CsNOD1-5 and CsNLR-C1/2 were ubiquitously expressed in various normal tissues. Bacterial infection with Vibro harveyi revealed distinct expression patterns of all the tested CsNLRs in gill, intestine, trunk kidney, liver and spleen. In particular, CsNOD1-4 and CsNLR-C2 were significantly upregulated in gills at 48 h post bacterial infection. In addition, CsNOD3 and CsNOD4 were significantly elevated in infectious intestine, trunk kidney, liver and spleen, revealing that their expressions were more sensitive to bacterial infection than other CsNLRs. Together with the computational protein-protein interaction network of CsNLRs, it was suggested that individual NLR genes had different roles in the innate immune cascades of C. semilaevi against bacterial infection. This study provides valuable information for further studies on CsNLR immune function.