Evaluation of physiological and biochemical responses in different seasons in Surti buffaloes
Evaluation of physiological and biochemical responses in different seasons in Surti buffaloes
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DOI：
10.14202/vetworld.2015.727-731
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发表时间：
2015-06-01
期刊：
影响因子：
1.6
通讯作者：
Patel, Pankaj A.
中科院分区：
文献类型：
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作者：
Chaudhary, Sandhya S.;Singh, Virendra Kumar;Patel, Pankaj A.
目的：研究炎热干燥、炎热潮湿和舒适季节对苏尔特水牛生理、血液、生化和氧化应激参数的影响。材料与方法：选取生理状态相近的泌乳苏提水牛10头。根据温湿指数（THI），将其自然暴露于环境的程度分为热干燥（THI 1），热潮湿（THI 2）和中度冬季/舒适季节（THI 3）。采集血液/血清样本，分析生理、血液学、生化和氧化应激参数。采用标准统计学方法对结果进行分析。结果:随着THI的升高，呼吸速率（RR）、红细胞（RBC）、红细胞压积（hematocrit）、血红蛋白（Hb）、平均细胞Hb浓度（MCHC）等生理参数、丙氨酸转氨酶（ALT）、Na、K、肌酐、血尿素氮、Mn、Cu、Zn等生化参数、皮质醇等激素、谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）等氧化应激参数等显著升高。脂质过氧化（LPO）和总抗氧化状态（TAS）以及葡萄糖、胆固醇和三碘甲状腺原氨酸（T-3）显著下降。结论：THI是水牛热应激的敏感指标，其受环境温度的影响大于相对湿度。THI升高与RR、总红细胞计数、Hb、红细胞压积、MCHC、ALT、尿素、钠、肌酐、三碘甲状腺原氨酸、SOD、GPx、LPO和TAS显著升高相关，与葡萄糖、胆固醇和三碘甲状腺原氨酸（T-3）显著降低相关。
Aim: This study was conducted to evaluate the impact of hot dry, hot humid and comfortable season on physiological, hematological, biochemical, and oxidative stress parameters in Surti buffaloes.Materials and Methods: Ten lactating Surti buffaloes of similar physiological status were selected. Based on the temperature-humidity index (THI), their natural exposure to the environment was categorized as hot dry (THI 1), hot humid (THI 2) and moderate winter/comfort season (THI 3). Blood/serum samples were collected and analyzed for physiological, hematological, biochemical, and oxidative stress parameters. The results were analyzed using standard statistical methods.Results: With increase in THI, significant rise in physiological parameters such as respiration rate (RR), hematological parameters such as red blood cell (RBC), hematocrit, hemoglobin (Hb) and mean cell Hb concentration (MCHC), biochemical parameters such as alanine aminotransferase (ALT), Na, K, creatinine, blood urea nitrogen, Mn, Cu and Zn, hormones such as cortisol and oxidative stress parameters such as glutathione peroxidase (GPx), superoxide dismutase (SOD), lipid peroxide (LPO) and total antioxidant status (TAS) and significant decline in glucose, cholesterol and triiodothyronine (T-3) was observed.Conclusion: It was concluded that THI is a sensitive indicator of heat stress and is impacted by ambient temperature more than the relative humidity in buffaloes. Higher THI is associated with significantly increased RR, total RBC count, Hb, hematocrit, MCHC, ALT, urea, sodium, creatinine, triiodothyronine, SOD, GPx, LPO and TAS and with significant decrease in glucose, cholesterol and triiodothyronine (T-3).