The untranslated regions of eukaryotic mRNAs: structure, function, evolution and bioinformatic tools for their analysis.
The untranslated regions of eukaryotic mRNAs: structure, function, evolution and bioinformatic tools for their analysis.
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DOI：
10.1093/bib/1.3.236
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发表时间：
2000-09-01
影响因子：
9.5
通讯作者：
Liuni, S
中科院分区：
文献类型：
--
作者：
Pesole, G;Grillo, G;Liuni, S
基因组的非编码部分的关键作用现在得到了广泛的承认。特别是，mRNA非翻译区参与了许多转录后调控途径，这些途径控制着mRNA的定位、稳定性和翻译效率。本文综述了近年来有关真核基因非翻译区功能特征的研究进展。为了能够对mRNA非翻译区(UTRs)进行系统和详细的序列分析，设计了一个非冗余的后生动物mRNA非翻译序列数据库UTRdb，用于注释特定功能元件的出现。这些要素的共识结构是在实验分析和比较分析的基础上设计出来的，已收集在UTRSite数据库中。设计了一个合适的模式匹配软件，用于在用户提交的序列中搜索UTRsite模式，并评估它们的统计意义。后生动物mRNAs未翻译序列的结构、组成和进化特征已被研究，呈现出独特的种内和种间模式。
The crucial role of the non-coding portion of genomes is now widely acknowledged. In particular, mRNA untranslated regions are involved in many post-transcriptional regulatory pathways that control mRNA localisation, stability and translation efficiency. A review is given of the most recent research works on the functional characterisation of eukaryotic mRNA untranslated regions. In order to make possible a systematic and detailed sequence analysis of mRNA untranslated regions (UTRs), a non-redundant database of metazoan mRNA untranslated sequences annotated for the occurrence of specific functional elements, UTRdb, was devised. These elements, whose consensus structure has been devised on the basis of experimental assays and of comparative analyses, have been collected in the UTRsite database. A suitable pattern-matching software has been devised to search UTRsite patterns in user-submitted sequences, also assessing their statistical significance. Structural, compositional and evolutionary features of untranslated sequences of metazoan mRNAs have been investigated showing peculiar intra- and interspecific patterns.