Platelet-rich plasma for tissue regeneration can be stored at room temperature for at least five days
Platelet-rich plasma for tissue regeneration can be stored at room temperature for at least five days
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DOI：
10.1080/09674845.2016.1233792
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发表时间：
2017-01-01
影响因子：
1.9
通讯作者：
Najafi, Mitra F.
中科院分区：
文献类型：
--
作者：
Moore, Gary W.;Maloney, James C.;Najafi, Mitra F.
背景：自体富血小板血浆（PRP）在包括牙科在内的各种临床环境中作为伤口愈合促进剂的使用越来越多。经常使用新鲜的PRP，需要每天抽吸。本研究通过在可变条件下储存PRP和评估生长因子释放作为持续生存能力的替代标记来研究使用储存的PRP而不必冷冻的可能性。方法：将新鲜提取的PRP分别保存于透氧容器和不透氧容器中，在加或不加前列腺素、间歇搅拌和不搅拌的条件下，持续搅拌8天。进行系列血小板计数、平均血小板体积、血小板分布宽度、血小板与大细胞比值及胶原诱导聚集。一旦胶原诱导的聚集完成，将血浆离心成血小板颗粒，分离上清并冷冻，用于批量分析释放的血小板衍生生长因子- bb （PDGF-BB）。结果：正如预期的那样，血小板计数、百分比聚集和PDGF-BB水平都随着时间的推移而降低。血小板参数表明，血小板在非透氧容器中更稳定，可能是由于pH漂移和在透氧容器中形成一定程度的微聚集体。结论：尽管血小板完整性和PDGF-BB随着时间的推移而下降，但间歇性搅拌的非透氧容器比其他储存条件保留了更好的血小板完整性和功能，PDGF-BB释放，具有5-8天的临床应用潜力。
Background: Autologous platelet-rich plasma (PRP) is gaining increasing use as a wound healing promoter in a variety of clinical settings, including dentistry. Fresh PRP is often used, necessitating daily draws. The present study investigates the possibility of using stored PRP without having to freeze it by storing PRP under variable conditions and assessing growth factor release as a surrogate marker of continued viability.Methods: Freshly drawn PRP was stored in oxygen permeable and non-oxygen permeable containers under conditions of constant agitation with or without added prostaglandin, intermittent agitation and no agitation, over an 8-day period. Serial platelet counts, mean platelet volume, platelet distribution width and platelet-large cell ratio, and collagen-induced aggregometry were undertaken. Once collagen-induced aggregation had gone to completion, the plasma was centrifuged to pellet platelet material and the supernatants separated and frozen for batched analysis of released platelet-derived growth factor-BB (PDGF-BB).Results: As would be anticipated, platelet counts, percentage aggregation and PDGF-BB levels all reduced over time. Platelet parameters suggested that platelets were more stable in the non-oxygen permeable containers, possibly due to pH drift and a degree of microaggregate formation in the oxygen permeable containers.Conclusion: Although platelet integrity and PDGF-BB fell over time, the intermittently agitated non-oxygen permeable container appeared to retain better platelet integrity and function, and PDGF-BB release, than other storage conditions, with potential for clinical use for 5-8days.