An efficient and robust continuous-flow bioreactor for the enzymatic preparation of phytosterol esters based on hollow lipase microarray.
An efficient and robust continuous-flow bioreactor for the enzymatic preparation of phytosterol esters based on hollow lipase microarray.
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DOI：
10.1016/j.foodchem.2021.131256
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发表时间：
2021-09
期刊：
影响因子：
8.8
通讯作者：
Liu Xu;Jing Wang;Fenghong Huang;Mingming Zheng
中科院分区：
文献类型：
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作者：
Liu Xu;Jing Wang;Fenghong Huang;Mingming Zheng
在这项研究中，开发了一种装有组织良好的脂肪酶微阵列的连续流生物反应器，用于可持续合成功能性脂质植物甾醇酯（PE）。制备了合适孔径的中空介孔硅球用于脂肪酶固定化，疏水修饰赋予脂肪酶优异的催化活性和稳定性。结果表明，紧密堆积的脂肪酶微阵列具有较大的比表面积，保证了连续流生物反应器中脂肪酶与底物之间的充分相互作用。同时，由于填料球的中空结构，底物可以以 1 mL/min 的速度通过反应器，转化率高达 93.6%。此外，反应器能够在连续 30 天的处理时间内生产 1564 克 PE/g 催化剂，这创下了 PE 合成的最高记录。这种可持续、高转化的流动系统为食品加工领域PE的规模化生产提供了可行的途径。
In this study, a continuous-flow bioreactor packed with well-organized lipase microarrays was developed for the sustainable synthesis of functional lipid-phytosterol esters (PEs). Hollow mesoporous silicon spheres with a suitable pore size were prepared for lipase immobilization, and the hydrophobic modification endowed the lipase with excellent catalytic activity and stability. The results showed that the condensely packed lipase microarrays offered large specific surface areas and guaranteed the thorough interaction between the lipase and substrates in the continuous-flow bioreactor. Meanwhile, the substrate could pass through the reactor at 1 mL/min with a high conversion of 93.6% due to the hollow structure of the packing spheres. Moreover, the reactors were able to produce 1564 g PEs/g catalyst in a continuous 30-day processing period, which set the highest records for PEs synthesis. This sustainable and highly-converting flow system provided a feasible path for scale-up production of PEs in the food processing area.