A role for transcription from a piRNA cluster in de novo piRNA production
A role for transcription from a piRNA cluster in de novo piRNA production
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DOI：
10.1261/rna.029777.111
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发表时间：
2012-02-01
期刊：
影响因子：
4.5
通讯作者：
Katsuma, Susumu
中科院分区：
文献类型：
--
作者：
Kawaoka, Shinpei;Mitsutake, Hiroshi;Katsuma, Susumu
piwi相互作用rna （piRNAs）是动物性腺中针对转座子的基于核酸的适应性免疫系统的核心。迄今为止，piRNA通路如何感知作为底物的元件以及如何启动新的piRNA生产仍然是难以捉摸的。在这里，通过利用GFP转基因，我们筛选并获得了克隆家蚕BmN4细胞系，这些细胞系产生大量扩增的GFP衍生的pirna，能够在反式中沉默GFP。在多个独立的细胞系中，通过piRNA途径沉默GFP表达，我们检测到来自内源性piRNA簇的共同转录物，其中该簇的一部分独特地与反义GFP序列融合。生物信息学分析表明，融合转录物是GFP主要pirna的来源。我们的数据表明，来自piRNA集群的转录在针对新插入启动从头piRNA生产中的作用。
PIWI-interacting RNAs (piRNAs) are at the heart of the nucleic acid-based adaptive immune system against transposons in animal gonads. To date, how the piRNA pathway senses an element as a substrate and how de novo piRNA production is initiated remain elusive. Here, by utilizing a GFP transgene, we screened and obtained clonal silkworm BmN4 cell lines producing massively amplified GFP-derived piRNAs capable of silencing GFP in trans. In multiple independent cell lines where GFP expression was silenced by the piRNA pathway, we detected a common transcript from an endogenous piRNA cluster, in which a part of the cluster is uniquely fused with an antisense GFP sequence. Bioinformatic analyses suggest that the fusion transcript is a source of GFP primary piRNAs. Our data implicate a role for transcription from a piRNA cluster in initiating de novo piRNA production against a new insertion.