Spinosad resistance of melon thrips, Thrips palmi, is conferred by G275E mutation in α6 subunit of nicotinic acetylcholine receptor and cytochrome P450 detoxification
Spinosad resistance of melon thrips, Thrips palmi, is conferred by G275E mutation in α6 subunit of nicotinic acetylcholine receptor and cytochrome P450 detoxification
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DOI：
10.1016/j.pestbp.2014.04.013
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发表时间：
2014-06-01
影响因子：
4.7
通讯作者：
Sonoda, Shoji
中科院分区：
文献类型：
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作者：
Bao, Wen Xue;Narai, Yutaka;Sonoda, Shoji
为了研究棕榈蓟马对多杀菌素的耐药机制，我们克隆了敏感菌株（OK）和耐药菌株（TSI和TS5）的烟碱乙酰胆碱受体α6亚基（TPα6）基因的部分核苷酸序列，并比较了三个菌株中推导的氨基酸序列。 OK、TS1和TS5菌株的LC50值分别为3.4 mg/L、2838.5 mg/L和6655.5 mg/L。推导的TPα6基因氨基酸序列与Frankliniella occidentalis的氨基酸序列有96%的一致性。比较3个菌株的TPα6基因氨基酸序列，发现TS1和TS5菌株在第275位氨基酸处有一个抗性氨基酸Glu，而OK菌株在相应的氨基酸位置上编码有一个敏感氨基酸Gly。增效剂胡椒基丁醚分别使OK、TS1和TS5菌株的耐药率降低1.1倍、5.8倍和9.0倍。这些结果表明，棕榈树对多杀菌素的抗性是由于 TP α 6 的敏感性降低和细胞色素 P450 介导的解毒作用而赋予的。 (C) 2014 Elsevier Inc. 保留所有权利。
To examine the resistance mechanisms of Thrips palmi against spinosad, we cloned partial nucleotide sequences of the nicotinic acetylcholine receptor alpha 6 subunit (TP alpha 6) gene from susceptible (OK) and resistant (TSI and TS5) strains and compared the deduced amino acid sequences among the three strains. The OK, TS1, and TS5 strains respectively showed LC50 values of 3.4 mg/L, 2838.5 mg/L, and 6655.5 mg/L. The deduced amino acid sequence of TP alpha 6 gene showed 96% identity with that of Frankliniella occidentalis. Comparison of the deduced amino acid sequences of TP alpha 6 gene among the three strains showed that the TS1 and TS5 strains had a resistant amino acid, Glu, at amino acid position 275. On the other hand, a susceptible amino acid, Gly, was encoded at the corresponding amino acid position for the OK strain. The synergist, piperonyl butoxide, respectively caused 1.1-fold, 5.8-fold, and 9.0-fold decreases in the resistance ratios of the OK, TS1, and TS5 strains. These results suggest that spinosad resistance of T. palmi is conferred by reduced sensitivity of TP alpha 6 and cytochrome P450-mediated detoxification. (C) 2014 Elsevier Inc. All rights reserved.