PREGNANCIES FOLLOWING THE FROZEN STORAGE OF EXPANDING HUMAN BLASTOCYSTS
PREGNANCIES FOLLOWING THE FROZEN STORAGE OF EXPANDING HUMAN BLASTOCYSTS
复制标题
DOI：
10.1007/bf01139337
复制
发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
FISHEL S B
中科院分区：
文献类型：
--
作者：
COHEN J;SIMONS R F;FISHEL S B
以甘油为冷冻保护剂，在含有丙酮酸和人血清的Earle‘s液中冷冻人类囊胚，并将其保存在液氮中。11例患者替换解冻胚泡，2例妊娠。一个怀孕的胚泡在被替换时正在孵化。有三个参数对胚胎存活和着床很重要：排卵到替换解冻囊胚的间隔时间，冷冻前胚胎发育良好的时间，以及开始降温时的胚泡发育阶段。
Human blastocysts were frozen in Earle's solution containing pyruvate and human serum, using glycerol as cryoprotectant and stored in liquid nitrogen. Thawed blastocysts were replaced in 11 patients, which resulted in 2 pregnancies. One blastocyst giving a pregnancy was hatching when replaced. Three parameters appeared to be important for embryo survival and implantation: the interval between ovulation and replacement of the thawed blastocysts, satisfactory embryonic development before freezing and the stage of blastulation when cooling began.