Human testicular peritubular cells secrete pigment epithelium-derived factor (PEDF), which may be responsible for the avascularity of the seminiferous tubules.
Human testicular peritubular cells secrete pigment epithelium-derived factor (PEDF), which may be responsible for the avascularity of the seminiferous tubules.
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DOI：
10.1038/srep12820
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发表时间：
2015-09-03
影响因子：
4.6
通讯作者：
Mayerhofer A
中科院分区：
文献类型：
--
作者：
Windschüttl S;Kampfer C;Mayer C;Flenkenthaler F;Fröhlich T;Schwarzer JU;Köhn FM;Urbanski H;Arnold GJ;Mayerhofer A
男性的生育能力取决于精子发生，精子发生在睾丸的曲细精管中。该隔室没有血管，然而血管存在于生精小管的壁中。我们的蛋白质组学研究使用培养的人睾丸管周细胞（HTPC），即细胞，形成这个壁，揭示了他们组成性分泌色素上皮衍生因子，PEDF，这是已知的发挥抗血管生成作用。免疫组织化学支持其在体内存在于人肾小管壁中。共培养研究和细胞迁移模式的分析表明，人内皮细胞（HUVECs）被HTPCs排斥。所涉及的因子可能是PEDF，因为PEDF抗血清阻断了排斥作用。因此，睾丸管周细胞，通过PEDF，可能会阻止人类曲细精管血管化。二氢睾酮（DHT）增加了HTPC中的PEDF（qPCR），但非人灵长类动物睾丸中的PEDF表达发生在青春期之前。因此，PEDF可能参与建立无血管性的曲细精管和青春期后雄激素可能进一步加强这一特点。睾丸微血管和血液流动有助于精原干细胞龛。因此，HTPC通过控制睾丸微血管可能有助于精原干细胞的调节，以及。
Male fertility depends on spermatogenesis, which takes place in the seminiferous tubules of the testis. This compartment is devoid of blood vessels, which are however found in the wall of the seminiferous tubules. Our proteomic study using cultured human testicular peritubular cells (HTPCs) i.e. the cells, which form this wall, revealed that they constitutively secrete pigment epithelium-derived factor, PEDF, which is known to exert anti-angiogenic actions. Immunohistochemistry supports its presence in vivo, in the human tubular wall. Co-culture studies and analysis of cell migration patterns showed that human endothelial cells (HUVECs) are repulsed by HTPCs. The factor involved is likely PEDF, as a PEDF-antiserum blocked the repulsing action. Thus testicular peritubular cells, via PEDF, may prevent vascularization of human seminiferous tubules. Dihydrotestosterone (DHT) increased PEDF (qPCR) in HTPCs, however PEDF expression in the testis of a non-human primate occurs before puberty. Thus PEDF could be involved in the establishment of the avascular nature of seminiferous tubules and after puberty androgens may further reinforce this feature. Testicular microvessels and blood flow are known to contribute to the spermatogonial stem cell niche. Hence HTPCs via control of testicular microvessels may contribute to the regulation of spermatogonial stem cells, as well.