Bioreactor design for propagation of somatic embryos
Bioreactor design for propagation of somatic embryos
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DOI：
10.1007/s11240-004-6647-0
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发表时间：
2005-06-01
影响因子：
3
通讯作者：
Heyerdahl, Petter H.
中科院分区：
文献类型：
--
作者：
Hvoslef-Eide, Anne Kathrine;Olsen, Odd Arild S.;Heyerdahl, Petter H.
挪威农业大学建造了六个相同的生物反应器，为植物细胞从细胞再生为体细胞胚胎(克隆或体细胞种子)提供了最佳条件。这是通过欧洲植物细胞培养物再生工作组在COST87中的合作得以实现的。生物反应器的设计通过一个低速搅拌器提供温和的搅拌，该搅拌器定期改变旋转方向，以防止悬浮液中细胞在其中定居和生长的“安静”区域。此外，氧气是通过硅胶细管环提供的，没有气泡，这些管环是松散的，与液体一起移动，以防止细胞在这些管上生长。我们尽可能使用现成的组件，将成本降低到最低，这是建造的另一个目标。结果是一套相对便宜的计算机控制的生物反应器，可以控制温度、氧气、pH、搅拌器速度和搅拌器方向。此外，我们还通过简单的滤光方式提供了不同的光谱质量。使用本软件，可以设置参数以在24小时的昼夜循环中每小时改变一次。与Erlenmeyer的相同培养物相比，我们所有的培养物在生物反应器中的生长都有所改善。他问道。所使用的培养物有：胡萝卜(Daucus Carota)、挪威云杉(Picea Abies)、白桦树(Betula Pendula)、仙客来(仙客来)的胚性培养和圣诞海棠(Begonia X Cheimantha)的嫩枝培养。文件还讨论了对现行制度的改进建议，以供今后修订。
Six identical bioreactors were constructed and built at the Agricultural University of Norway to provide optimal conditions for plant cell regeneration from cells into somatic embryos ('clonal or somatic seeds'). This was made possible through cooperation in COST87 by a European network in a working group on regeneration from plant cell cultures. The bioreactor design provides gentle stirring through a slow-speed stirrer that regularly changes direction of rotation to prevent 'quiet' zones in the suspension in which cells can settle and grow. In addition, the oxygen is provided, bubble-free, through thin silicone tubing loops that are hanging loose, moving with the liquid to prevent cell growth on these tubes. We used off-the-shelf components whenever possible, to reduce the costs to a minimum, which was another aim of the construction. The result was a suite of relatively inexpensive computer-controlled bioreactors that could control temperature, oxygen, pH, stirrer speed and stirrer direction. In addition, we have provided different light spectral qualities by simple means of filtering the light. Using the present software, the parameters can be set up to alter every hour during the 24-h day/night cycle. All our cultures have improved growth in the bioreactors compared to identical cultures in Erlenmeyer. asks. The cultures used are: embryogenic cultures of carrot (Daucus carota), Norway spruce (Picea abies), birch (Betula pendula), cyclamen (Cyclamen persicum) and shoot cultures of Christmas begonia (Begonia x cheimantha). The paper also discusses recommendations for improvements of the current system for future revisions.