Gram-negative bacteria produce membrane vesicles which are capable of killing other bacteria
Gram-negative bacteria produce membrane vesicles which are capable of killing other bacteria
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DOI：
10.1128/jb.180.20.5478-5483.1998
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发表时间：
1998-10-01
影响因子：
3.2
通讯作者：
Beveridge, TJ
中科院分区：
文献类型：
--
作者：
Li, ZS;Clarke, AJ;Beveridge, TJ
从15种革兰氏阴性菌（柠檬酸杆菌属、肠杆菌属、埃希氏菌属、克雷伯氏菌属、摩根氏菌属、变形杆菌属、沙门氏菌属和志贺氏菌属菌株）中分离的天然产生的膜囊泡（MV）裂解了许多革兰氏阳性菌（包括分枝杆菌）和革兰氏阴性菌培养物。肽聚糖酶谱表明MV含有肽聚糖水解酶，电子显微镜显示囊状胞壁蛋白被消化，证实了先前的操作方式（J. L. Kadurugamuwa和T. J. Beveridge，J. Bacteriol. 174：2767-2774，1996）。MV敏感细菌具有A1 α、A4 α、A1 γ、A2 α和A4 γ肽聚糖化学型，而A3 α、A3 β、A3 γ、A4 β、B1 α和B1 β化学型不受影响。铜绿假单胞菌PAO 1囊泡具有最强的溶解活性。
Naturally produced membrane vesicles (MVs), isolated from 15 strains of gram-negative bacteria (Citrobacter, Enterobacter, Escherichia, Klebsiella, Morganella, Proteus, Salmonella, and Shigella strains), lysed many gram-positive (including Mycobacterium) and gram-negative cultures. Peptidoglycan zymograms suggested that MVs contained peptidoglycan hydrolases, and electron microscopy revealed that the murein sacculi were digested, confirming a previous modus operandi (J. L. Kadurugamuwa and T. J. Beveridge, J. Bacteriol. 174: 2767-2774, 1996). MV-sensitive bacteria possessed A1 alpha, A4 alpha, Al gamma, A2 alpha, and A4 gamma peptidoglycan chemotypes, whereas A3 alpha, A3 beta, A3 gamma, A4 beta, B1 alpha, and B1 beta chemotypes were not affected. Pseudomonas aeruginosa PAO1 vesicles possessed the most lytic activity.