A fructose/H(+) symporter controlled by a LacI-type regulator promotes survival of pandemic Vibrio cholerae in seawater.
A fructose/H(+) symporter controlled by a LacI-type regulator promotes survival of pandemic Vibrio cholerae in seawater.
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由 LacI 型调节因子控制的果糖/H( ) 同向转运蛋白可促进大流行性霍乱弧菌在海水中的存活。
DOI：
10.1038/s41467-021-24971-3
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发表时间：
2021-07-30
影响因子：
16.6
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Liu Y;Liu B;Xu T;Wang Q;Li W;Wu J;Zheng X;Liu B;Liu R;Liu X;Guo X;Feng L;Wang L
霍乱弧菌（Vibrio cholesterol）是一种寄生于人类肠道的细菌，它的大部分生命周期都在海水中度过。当病原体在海水和人体肠道之间移动时，必须适应大量的环境变化，包括果糖等碳源的不同可用性。在这里，我们使用体外实验以及小鼠肠道定植试验来研究流行性霍乱弧菌适应这些环境变化的机制。我们表明，一个LacI型调节器（FruI）和果糖/H+同向转运体（FruT）是重要的果糖摄取在低果糖浓度，如海水中发现的。FruT被FruI下调，当O2浓度低时（如在肠道中），FruI被ArcAB上调，ArcAB是一种已知对氧气水平变化做出反应的双组分系统。因此，细菌主要使用FruT在海水条件下（低果糖，高O2）摄取果糖，并使用已知的果糖磷酸转移酶系统（PTS，Fpr）在肠道中发现的条件下摄取果糖。PTS活性导致细胞内cAMP水平降低，从而上调毒力基因。我们的研究结果表明，FruT/FruI系统可能是重要的流行性霍乱弧菌在海水中的生存。霍乱弧菌（Vibrio cholesterol）是一种寄生于人类肠道的细菌，它的大部分生命周期都在海水中度过。在这里，Liu等人确定了一个果糖摄取系统，该系统对海水中细菌的生长很重要，海水中果糖浓度很低。
The bacterium Vibrio cholerae can colonize the human intestine and cause cholera, but spends much of its life cycle in seawater. The pathogen must adapt to substantial environmental changes when moving between seawater and the human intestine, including different availability of carbon sources such as fructose. Here, we use in vitro experiments as well as mouse intestinal colonization assays to study the mechanisms used by pandemic V. cholerae to adapt to these environmental changes. We show that a LacI-type regulator (FruI) and a fructose/H+ symporter (FruT) are important for fructose uptake at low fructose concentrations, as those found in seawater. FruT is downregulated by FruI, which is upregulated when O2 concentrations are low (as in the intestine) by ArcAB, a two-component system known to respond to changes in oxygen levels. As a result, the bacteria predominantly use FruT for fructose uptake under seawater conditions (low fructose, high O2), and use a known fructose phosphotransferase system (PTS, Fpr) for fructose uptake under conditions found in the intestine. PTS activity leads to reduced levels of intracellular cAMP, which in turn upregulate virulence genes. Our results indicate that the FruT/FruI system may be important for survival of pandemic V. cholerae in seawater. The bacterium Vibrio cholerae can colonize the human intestine and cause cholera, but spends much of its life cycle in seawater. Here, Liu et al. identify a fructose uptake system that is important for the growth of the bacteria in seawater, where fructose concentrations are low.
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