Flo11p adhesin required for meiotic differentiation in Saccharomyces cerevisiae minicolonies grown on plastic surfaces.
Flo11p adhesin required for meiotic differentiation in Saccharomyces cerevisiae minicolonies grown on plastic surfaces.
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DOI：
10.1111/j.1567-1364.2010.00712.x
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发表时间：
2011-03
影响因子：
3.2
通讯作者：
Honigberg SM
中科院分区：
文献类型：
--
作者：
White MG;Piccirillo S;Dusevich V;Law DJ;Kapros T;Honigberg SM
在塑料表面生长的酿酒酵母形成有组织的结构，称为小菌落，由圆形（酵母样）细胞核心组成，周围环绕着丝状细胞链（假菌丝）。与在同一表面上生长的非结构化酵母菌群相比，小菌落对塑料的亲和力要高得多。这些菌落表面的假菌丝进一步发育成子囊链。这些结构表明假菌丝分化和孢子形成是小菌落中的连续过程。与这个想法一致，在刺激假菌丝分化的条件下生长的小菌落含有更高频率的子囊。此外，无法形成假菌丝的 flo11Δ 突变体在培养物中产生正常的孢子形成，但在小集落孢子形成方面存在缺陷。当小菌落分散在水中并且细胞随后沉降在塑料表面时，这些细胞非常有效地形成孢子。综上所述，我们的结果表明，小菌落中的孢子形成受到假菌丝分化的刺激，因为这些假菌丝从菌落的核心分散。
S. cerevisiae grown on plastic surfaces formed organized structures, termed minicolonies, that consisted of a core of round (yeast-like) cells surrounded by chains of filamentous cells (pseudohyphae). Minicolonies had much higher affinity for plastic than unstructured yeast communities growing on the same surface. Pseudohyphae at the surface of these colonies developed further into chains of asci. These structures suggest that pseudohyphal differentiation and sporulation are sequential processes in minicolonies. Consistent with this idea, minicolonies grown under conditions that stimulated pseudohyphal differentiation contained higher frequencies of asci. Furthermore, a flo11Δ mutant, which fails to form pseudohyphae, yielded normal sporulation in cultures but was defective for minicolony sporulation. When minicolonies were dispersed in water and cells then allowed to settle on the plastic surface, these cells sporulated very efficiently. Taken together, our results suggest that sporulation in minicolonies is stimulated by pseudohyphal differentiation because these pseudohyphae are dispersed from the core of the colony.