Metabolomic Study of Interactive Effects of Phenol, Furfural, and Acetic Acid on Saccharomyces cerevisiae
Metabolomic Study of Interactive Effects of Phenol, Furfural, and Acetic Acid on Saccharomyces cerevisiae
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苯酚、糠醛和乙酸对酿酒酵母相互作用影响的代谢组学研究
DOI：
10.1089/omi.2011.0003
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发表时间：
2011-10-01
影响因子：
3.3
通讯作者：
Yuan, Ying-Jin
中科院分区：
文献类型：
--
作者：
Ding, Ming-Zhu;Wang, Xin;Yuan, Ying-Jin
采用代谢分析法研究了糠醛、苯酚和乙酸3种木质纤维素水解液中的典型抑制剂对酿酒酵母乙醇发酵的影响。结果表明，三种抑制剂对酵母菌的生长、发酵和某些代谢产物具有明显的协同作用。在三种抑制剂的组合中，乙酸对酵母的影响最大，它促进了氨基酸代谢，抑制了中心碳代谢。苯酚和糠醛的存在增强了醋酸对酵母细胞的影响，这可能是由于膜完整性的丧失和代谢的抑制。进一步的研究表明，抑制剂的组合也表现出拮抗作用，主要是对苏氨酸，尸胺，肌醇，色氨酸，削弱或逆转个别抑制剂的效果。这可能是由于联合抑制剂对细胞的损伤更严重，以及单独和联合抑制剂存在时细胞的修复机制不同。深入了解这些抑制剂的协同和拮抗作用，将有助于抗性菌株的改良和木质纤维素发酵工艺的优化。
Metabolic profiling was carried out to investigate the interactive effects of three representative inhibitors (furfural, phenol, and acetic acid) in lignocellulosic hydrolysate on Saccharomyces cerevisiae during ethanol fermentation. Our results revealed that three inhibitors exhibited significantly synergistic effects on the growth, fermentation, and some metabolites of yeast. Acetic acid exerted the most severe effects on yeast in the combination of three inhibitors, enhancing amino acids metabolism and inhibiting central carbon metabolism. The effects on yeast cells by acetic acid were enhanced by the presence of phenol and furfural, which might be owing to the loss of membrane integrity and the inhibition on metabolism. Further investigation indicated that the combination of inhibitors also exhibited antagonistic effects mainly on threonine, cadaverine, inositol, and tryptophan, weakening or reversing the effects of individual inhibitor. It might be due to the more severe damage by the combined inhibitors, and different repairing mechanism of cells in the presence of individual and combined inhibitors. Better understanding of the synergistic and antagonistic effects of the inhibitors will be helpful for the improvement of tolerant strains and the optimization of lignocellulosic fermentation.