Microchip-based immunoassay system with branching multichannels for simultaneous determination of interferon-γ
Microchip-based immunoassay system with branching multichannels for simultaneous determination of interferon-γ
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DOI：
10.1002/1522-2683(200203)23:5
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发表时间：
2002-03-01
期刊：
影响因子：
2.9
通讯作者：
Kitamori, T
中科院分区：
文献类型：
--
作者：
Sato, K;Yamanaka, M;Kitamori, T
构建了一种适用于多样品同时检测的微芯片珠床免疫分析系统。该芯片具有分支多通道和四个反应检测区；所构建的系统可以一次处理四个样品，只需要一个泵单元。干扰素γ采用三步夹心免疫分析法，系统与热透镜显微镜耦合作为检测器。从每个通道获得的信号强度偏差在10%以内，变异系数几乎与单一直通道试验相同。在单通道检测中，四个样品的检测时间为50分钟，而不是一个样品的35分钟；因此，采用分支结构芯片实现了更高的吞吐量。
A bead-bed immunoassay system suitable for simultaneous assay of multiple samples was constructed on a microchip. The chip had branching multichannels and four reaction and detection regions; the constructed system could process four samples at a time with only one pump unit. Interferon gamma was assayed by a 3-step sandwich immunoassay with the system coupled to a thermal lens microscope as a detector. The biases of the signal intensities obtained from each channel were within 10%, and coefficients of variation were almost the same level as the single straight channel assay. The assay time for four samples was 50 min instead of 35 min for one sample in the single-channel assay; hence higher throughput was realized with the branching structure chip.