A method of cultivating myx-omycete plasmodia
A method of cultivating myx-omycete plasmodia
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DOI：
10.2307/2480903
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发表时间：
1936-01-01
期刊：
影响因子：
--
通讯作者：
CAMP, W. G.
中科院分区：
文献类型：
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作者：
CAMP, W. G.
本文描述了一种在湿室中用倒置的培养皿支撑的纱布或纸的底层上培养多头绒泡菌原虫的简单方法。水通过纱布或纸的芯子作用从腔体底部供应给原虫。碾磨成粉的燕麦被用作食物，随着粘菌的利用，每天添加的量越来越多。多余的原质可以去除并用于实验，也可以慢慢风干成菌核。菌核在暴露在液态空气中10分钟或暴露在52摄氏度下两周后仍然存活。建议使用原生质团进行进一步的化学、物理和生物实验。关于原生质的研究。|摘要作者：F.L.霍华德
A simple method is described for growing plasmodium of Physarum polycephalum on a sub-stratum of gauze or paper supported by an inverted Petri dish in a moist chamber. Water is supplied the plasmodium by wick action of the gauze or paper from the bottom of the chamber. Pulverized rolled oats are supplied as food and daily increasingly larger amounts are added as it is utilized by the myxomycete. Excess plasmodium may be removed and used for experiments or slowly air-dried into sclerotia. Sclerotia remain viable after 10 minutes' exposure to liquid air or two weeks' exposure to 52[degree] C. The use of plasmodium is suggested for further chemical, physical, and biol. research on protoplasm. || ABSTRACT AUTHORS: F. L. Howard