Vascularized 3D printed scaffolds for promoting bone regeneration
Vascularized 3D printed scaffolds for promoting bone regeneration
复制标题
用于促进骨再生的血管化 3D 打印支架。
DOI：
10.1016/j.biomaterials.2018.10.033
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发表时间：
2019-01-01
期刊：
影响因子：
14
通讯作者：
Deng, Lianfu
中科院分区：
文献类型：
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作者：
Yan, Yufei;Chen, Hao;Deng, Lianfu
3D打印支架在骨组织再生方面具有很好的前景。受骨发育阶段过程的启发，3D打印支架具有快速的内部血管形成能力和强大的骨诱导生物活性，将成为临床使用的理想骨替代品。在这里，我们制造了一个3D打印的可生物降解支架，可以控制释放去铁胺，通过表面氨解和逐层组装技术，这对于血管生成和成骨至关重要，并与骨骼发育和重建相匹配。我们的体外研究表明，支架可显著加速人脐带内皮细胞血管模式的形成，促进矿化基质的产生，促进间充质干细胞成骨分化过程中成骨相关基因的表达。体内实验结果表明，去铁胺能促进大鼠骨缺损模型缺损部位血管长入，促进骨再生。此外，该3d打印支架具有良好的生物相容性，适合间充质干细胞生长和分化，并具有类似于天然松质骨的适当力学性能。综上所述，该3d打印支架具有灵活性、经济性和实用性，在临床移植治疗节段性骨缺损方面具有巨大的潜力。
3D printed scaffolds hold promising perspective for bone tissue regeneration. Inspired by process of bone development stage, 3D printed scaffolds with rapid internal vascularization ability and robust osteoinduction bioactivity will be an ideal bone substitute for clinical use. Here, we fabricated a 3D printed biodegradable scaffold that can control release deferoxamine, via surface aminolysis and layer-by-layer assembly technique, which is essential for angiogenesis and osteogenesis and match to bone development and reconstruction. Our in vitro studies show that the scaffold significantly accelerates the vascular pattern formation of human umbilical endothelial cells, boosts the mineralized matrix production, and the expression of osteogenesis-related genes during osteogenic differentiation of mesenchymal stem cells. In vivo results show that deferoxamine promotes the vascular ingrowth and enhances the bone regeneration at the defect site in a rat large bone defect model. Moreover, this 3D-printed scaffold has excellent biocompatibility that is suitable for mesenchymal stem cells grow and differentiate and possess the appropriate mechanical property that is similar to natural cancellous bone. In summary, this 3D-printed scaffold holds huge potential for clinical translation in the treatment of segmental bone defect, due to its flexibility, economical friendly and practicality.