Mass spectrometric identification of a phorbol diester 12-O-hexadecanoylphorbol-13-acetate, an Epstein-Barr virus-activating substance, in the soil collected from under Sapium sebiferum.
Mass spectrometric identification of a phorbol diester 12-O-hexadecanoylphorbol-13-acetate, an Epstein-Barr virus-activating substance, in the soil collected from under Sapium sebiferum.
复制标题
对从乌桕下收集的土壤中的佛波二酯 12-O-十六酰佛波醇-13-乙酸酯（一种 Epstein-Barr 病毒激活物质）进行质谱鉴定。
DOI：
10.1016/0304-3835(91)90180-p
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发表时间：
1991
期刊：
影响因子：
9.7
通讯作者：
F. Mizuno
中科院分区：
文献类型：
--
作者：
N. Takeda;H. Ohigashi;N. Hirai;K. Koshimįzu;M. Suzuki;A. Tatematsu;T. Osato;F. Mizuno
几种大戟科植物的土壤提取物中含有EB病毒（Epstein-Barr virus，EBV）激活物质，该物质被认为是引起中国南方鼻咽癌（NPC）和非洲热带地区伯基特淋巴瘤（Burkitt's lymphoma，BL）的环境辅助因子之一。然后，进行了一个模型实验，旨在化学特性的活性物质，使用周围的土壤提取物乌桕，日本代表大戟科植物。由EBV早期抗原（EA）诱导活性引导的色谱分离得到高活性级分。将该馏分应用于解吸化学电离质谱法，鉴定出主要活性物质是12-O-十六酰基佛波醇-13-乙酸酯（HPA），它最初存在于这种植物中。本模型试验的方法可推广应用于其他鼻咽癌和BL流行区的样品。
Soil-extracts collected from the ground from under several Euphorbiaceae plants have been known to possess Epstein-Barr virus (EBV)-activating substances which are thought to be one of the environmental co-factors causing nasopharyngeal carcinoma (NPC) in southern part of China and Burkitt's lymphoma (BL) in tropical Africa. Then, a model experiment aimed at chemical characterization of such active substances was carried out using a soil-extract around Sapium sebiferum, a Japanese representative Euphorbiaceae plant. Chromatographic separation guided by the EBV early antigen (EA) inducing activity gave a highly active fraction. Application of this fraction to desorption chemical ionization mass spectrometry identified a major active substance to be 12-O-hexadecanoylphorbol-13-acetate (HPA), which originally occurs in this plant. The method in this model experiment is suggested to be applicable to other samples from the endemic areas of NPC and BL.