Morphological development of thick-tufted layer v pyramidal cells in the rat somatosensory cortex.
Morphological development of thick-tufted layer v pyramidal cells in the rat somatosensory cortex.
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大鼠体感皮质中厚的层V锥体细胞的形态发展。
DOI：
10.3389/fnana.2011.00005
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发表时间：
2011
影响因子：
2.9
通讯作者：
Markram H
中科院分区：
文献类型：
--
作者：
Romand S;Wang Y;Toledo-Rodriguez M;Markram H
厚簇状V层锥体神经元(TTL5)是提供新皮质输出的关键神经元。虽然已经进行了广泛的研究，但在发育过程中支配其树突和轴突树枝的原理仍然没有完全量化。本研究利用大鼠体感觉皮层中的Bioctin标记细胞重建的三维模型神经元，对出生后7、14、21、36和60天的TTL5细胞进行了详细的形态分析。揭示了三个发育阶段，不同发育区段的生长速率和变化特征不同。从P7到P14，几乎所有的分室都生长迅速，沿着顶端树突的丝足状节段消失；从P14到P21，生长局限于每个分室的特定节段，棘突和脊突的密度显著增加；从P21到P60，基本树突节段在特定的分枝顺序上显著增加，部分基树突节和斜树突节延长或增粗。因此，发育变化表现为两种模式：第一阶段总体快速增长，随后阶段局部增长缓慢(主要在中间体上增厚或主要在末端加长)。延长可能伴随着不同节段的回缩。这些结果揭示了发育过程中神经元隔间树状结构的不同调节，支持功能隔间发育的概念。这一量化为TTL5形态在信息处理以及其他目的中的潜在价值提供了新的见解。
The thick-tufted layer V pyramidal (TTL5) neuron is a key neuron providing output from the neocortex. Although it has been extensively studied, principles governing its dendritic and axonal arborization during development are still not fully quantified. Using 3-D model neurons reconstructed from biocytin-labeled cells in the rat somatosensory cortex, this study provides a detailed morphological analysis of TTL5 cells at postnatal day (P) 7, 14, 21, 36, and 60. Three developmental periods were revealed, which were characterized by distinct growing rates and properties of alterations in different compartments. From P7 to P14, almost all compartments grew fast, and filopodia-like segments along apical dendrite disappeared; From P14 to P21, the growth was localized on specified segments of each compartment, and the densities of spines and boutons were significantly increased; From P21 to P60, the number of basal dendritic segments was significantly increased at specified branch orders, and some basal and oblique dendritic segments were lengthened or thickened. Development changes were therefore seen in two modes: the fast overall growth during the first period and the slow localized growth (thickening mainly on intermediates or lengthening mainly on terminals) at the subsequent stages. The lengthening may be accompanied by the retraction on different segments. These results reveal a differential regulation in the arborization of neuronal compartments during development, supporting the notion of functional compartmental development. This quantification provides new insight into the potential value of the TTL5 morphology for information processing, and for other purposes as well.